Rabbit Anti-MYL2 Recombinant Antibody (VS13-YC760)
CAT#: VS13-YC760
The Rabbit Anti-MYL2 Recombinant Antibody is specific for human, mouse and rat MYL2. MYL2 encodes a regulatory light chain in cardiac myosin, crucial for contraction mechanisms via phosphorylation. The anti-MYL2 antibody can be used for Western blot (1/500-1/5000), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, HSkMC, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
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Specifications
- Immunogen
- Recombinant human Myosin Light Chain 2, aa 117-166.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYL2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Myosin Light Chain 2; Myosin Light Chain 2, Slow Skeletal/Ventricular Muscle Isoform; Myosin, Light Polypeptide 2, Regulatory, Cardiac, Slow; Myosin, Light Chain 2, Regulatory, Cardiac, Slow; Cardiac Ventricular Myosin Light Chain 2; Ventricular Myosin Light Chain 2; Cardiac Myosin Light Chain 2; MLC-2s/V; MLC-2v;
- Gene ID
- 4633
- UniProt ID
- P10916
- Long Name
- Myosin Light Chain 2
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_034991.3
- Function
- Contractile protein that plays a role in heart development and function (PubMed:10409661).
Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661).
During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MYL2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2241MZ | Recombinant Mouse Anti-Human MYL2 Antibody | WB | Mouse antibody |
| MRO-1057-CN | Recombinant Rabbit Anti-MYL2 Monoclonal Antibody (CBACN-391) | WB, IHC, IP | Rabbit IgG |
| MRO-2053-CN | Rabbit Anti-MYL2 Polyclonal Antibody (MRO-2053-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ1021 | Rabbit Anti-MYL2 Recombinant Antibody (VS3-CJ1021) | WB, IHC, IP | Rabbit IgG |
| VS-0223-WK47 | Rabbit Anti-MYL2 Recombinant Antibody (VS-0223-WK47) | WB, ICC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2345 | Hi-Affi™ Recombinant Rabbit Anti-MYL2 Monoclonal Antibody (DS2345AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1420 | Anti-MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4641 | Anti-Mouse MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4640 | Anti-Human MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4642 | Anti-Rat MYL2 Immunohistochemistry Kit | IHC |
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