Rabbit Anti-MYL2 Recombinant Antibody (VS13-YC760) (CAT#: VS13-YC760)
The Rabbit Anti-MYL2 Recombinant Antibody is specific for human, mouse and rat MYL2. MYL2 encodes a regulatory light chain in cardiac myosin, crucial for contraction mechanisms via phosphorylation. The anti-MYL2 antibody can be used for Western blot (1/500-1/5000), Immunohistochemistry-paraffin (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
Figure 2 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
Figure 6 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, HSkMC, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
Specifications
- Immunogen
- Recombinant human Myosin Light Chain 2, aa 117-166.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYL2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Myosin Light Chain 2; Myosin Light Chain 2, Slow Skeletal/Ventricular Muscle Isoform; Myosin, Light Polypeptide 2, Regulatory, Cardiac, Slow; Myosin, Light Chain 2, Regulatory, Cardiac, Slow; Cardiac Ventricular Myosin Light Chain 2; Ventricular Myosin Light Chain 2; Cardiac Myosin Light Chain 2; MLC-2s/V; MLC-2v;
- Gene ID
- 4633
- UniProt ID
- P10916
- Long Name
- Myosin Light Chain 2
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_034991.3
- Function
- Contractile protein that plays a role in heart development and function (PubMed:10409661).
Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661).
During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2345 | Hi-Affi™ Recombinant Rabbit Anti-MYL2 Monoclonal Antibody (DS2345AB) | WB, IHC | IgG |
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