Mouse Anti-MYLK Recombinant Antibody (clone 7F2B11)
CAT#: VS7-0425-WR451
This product is a mouse IgG1 antibody that binds to human MYLK. The antibody 7F2B11 recognizes specific amino acid domain of human MYLK (AA:1375-1524). It can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/1742_C2_13_cr58048fbf3cbb5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48055/1742_D1_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_A_7_3.jpg
Normal Tissue
Figure 5 High expression in smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/88290_B_5_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065534-MYLK
❮
❯
Specifications
- Immunogen
- Recombinant human MYLK (AA:1375-1524) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MYLK
- Species Reactivity
- Human
- Clone
- 7F2B11
- Molecule Class
- Calcium/Calmodulin-Dependent Protein Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Aortic aneurysm, familial thoracic 7 (AAT7); Megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MYLK; MLCK1; Telokin; KRP; MYLK1; Smooth Muscle Myosin Light Chain Kinase; MSTP083; SmMLCK; MLCK; EC 2.7.11.18; Kinase-Related Protein; AAT7; Myosin Light Chain Kinase; Myosin, Light Polypeptide Kinase; MLCK210; Myosin Light Chain Kinase, Smooth Muscle
- Gene ID
- 4638
- UniProt ID
- Q15746
- Long Name
- Myosin light chain kinase
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton
- Post Translation Modifications
- Acetylation; Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_001308238.1
- Function
- Actin-binding; Calmodulin-binding; Kinase; Serine/threonine-protein kinase; Transferase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR451. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MYLK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3130z | Mouse Anti-MYLK Recombinant Antibody (clone 1H10) | WB, ELISA | Mouse IgG1, κ |
| MOB-1038MZ | Recombinant Mouse Anti-Human MYLK Antibody | WB | Mouse antibody |
| MRO-2052-CN | Rabbit Anti-MYLK Polyclonal Antibody (MRO-2052-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-WK996 | Rabbit Anti-MYLK Recombinant Antibody (VS3-WK996) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS4-CJ48 | Recombinant Rabbit Anti-MYLK Antibody (clone 28C5) | ELISA, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0762MZ | Chicken Anti-MYLK Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2349 | Hi-Affi™ Recombinant Rabbit Anti-MYLK Monoclonal Antibody (DS2349AB) | WB, FC, ICC, IF, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1426 | Anti-MYLK Immunohistochemistry Kit | IHC | |
| VS-0525-XY4648 | Anti-Human MYLK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC762 | CytoStream™ Rabbit Anti-MYLK Recombinant Antibody (VS13-YC762) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, IF, IP, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
