Mouse Anti-MYLK Recombinant Antibody (clone 7F2B11) (CAT#: VS7-0425-WR451)
This product is a mouse IgG1 antibody that binds to human MYLK. The antibody 7F2B11 recognizes specific amino acid domain of human MYLK (AA:1375-1524). It can be used for WB, IHC, ICC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/1742_C2_13_cr58048fbf3cbb5_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/1742_C2_13_cr58048fbf3cbb5_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48055/1742_D1_4_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48055/1742_D1_4_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_A_7_3.jpg
Figure 5 High expression in smooth muscle
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/88290_B_5_7.jpg
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/88290_B_5_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065534-MYLK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065534-MYLK
Specifications
- Immunogen
- Recombinant human MYLK (AA:1375-1524) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MYLK
- Species Reactivity
- Human
- Clone
- 7F2B11
- Molecule Class
- Calcium/Calmodulin-Dependent Protein Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Aortic aneurysm, familial thoracic 7 (AAT7); Megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MYLK; MLCK1; Telokin; KRP; MYLK1; Smooth Muscle Myosin Light Chain Kinase; MSTP083; SmMLCK; MLCK; EC 2.7.11.18; Kinase-Related Protein; AAT7; Myosin Light Chain Kinase; Myosin, Light Polypeptide Kinase; MLCK210; Myosin Light Chain Kinase, Smooth Muscle
- Gene ID
- 4638
- UniProt ID
- Q15746
- Long Name
- Myosin light chain kinase
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton
- Post Translation Modifications
- Acetylation; Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_001308238.1
- Function
- Actin-binding; Calmodulin-binding; Kinase; Serine/threonine-protein kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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