Rabbit Anti-NAMPT Recombinant Antibody (VS3-FY2817) (CAT#: VS3-FY2817)
This anti-NAMPT antibody is a rabbit recombinant monoclonal antibody. It has been specifically designed to target the human NAMPT protein. It cross-reacts with mouse, rat species. The monoclonal antibody can be used in the area of Aging and Gestational Diabetes. This monoclonal antibody is suitable for employment in Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57722/1043_H9_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57722/1043_H9_2_selected.jpg
Figure 2 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/ihc_selected.jpg
Figure 3 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells of white and red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34349/157328_B_7_4_selected.jpg
(Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells of white and red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34349/157328_B_7_4_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_7_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_5_6.jpg
Figure 6 Appendix
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Lymphoid tissue Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_3_2.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Lymphoid tissue Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47776/114368_A_3_2.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105835-NAMPT
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105835-NAMPT
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NAMPT
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Aging and Gestational Diabetes
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Nicotinamide Phosphoribosyltransferase; Visfatin; Pre-B-Cell Colony-Enhancing Factor 1; Pre-B Cell-Enhancing Factor; EC 2.4.2.12; NAmPRTase; PBEF1
- Gene ID
- 10135
- UniProt ID
- P43490
- Sequence Similarities
- Belongs to the NAPRTase family.
- Cellular Localization
- Nucleus
Cytoplasm
Secreted
- Post Translation Modifications
- Ubiquitination at Lys99, Lys189, Lys342, and Lys371 (NX_P43490 [NX_P43490-1])
Modification sites at PhosphoSitePlus (P43490)
Glycosylation from GlyGen (P43490) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047275656.1; XP_054212959.1; XP_054212960.1; NP_005737.1
- Function
- Plays a role in the modulation of circadian clock function.
NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1559CT | Recombinant Mouse anti-Human NAMPT Monoclonal antibody (5E6) | ELISA, WB | |
| ZG-0358C | Mouse Anti-NAMPT Recombinant Antibody (ZG-0358C) | WB, ELISA | Mouse IgG |
| ZG-0359C | Mouse Anti-NAMPT Recombinant Antibody (ZG-0359C) | WB, ELISA | Mouse IgG |
| VS3-QX799 | Mouse Anti-NAMPT Recombinant Antibody (clone 2E11-F7-G3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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