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    Rabbit Anti-NCL Recombinant Antibody (VS13-YC770) (CAT#: VS13-YC770)

    The Rabbit Anti-NCL Recombinant Antibody is specific for human, mouse and rat NCL. NCL encodes a nucleolar phosphoprotein, integral to ribosomal synthesis, spanning 14 exons, with small nucleolar RNA encoded within an intron. The anti-NCL antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/200), Immunohistochemistry-paraffin (1/200-1/400).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/if_selected.jpg
    Figure 2 IF staining of human cell line HEL
    Figure 2 IF staining of human cell line HEL
    (Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, nucleoli rim & mitotic chromosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71110/1925_C12_8_cr5ed0fca081eb8_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli rim.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/669_C1_1_selected.jpg
    Figure 4 IHC staining of human duodenum
    Figure 4 IHC staining of human duodenum
    (Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/ihc_selected.jpg
    Figure 5 IHC staining of human colon
    Figure 5 IHC staining of human colon
    (Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/ihc_selected.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_B_7_5.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_9_4.jpg
    Figure 8 Pancreas
    Figure 8 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/19179_A_2_3.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_8_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Leydig cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Peritubular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Round or early spermatids
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Sertoli cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_6_6.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_7_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115053-NCL

    Specifications

    • Immunogen
    • Recombinant human CSDE1, aa 661-710.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat NCL
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, ICC, IF, IHC-P
    • Conjugate
    • NONE

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipping with ice pack.

    Applications

    • Application Notes
    • Western blot (WB): 1/500-1/5000
      Immunocytochemistry (ICC): 1/200
      Immunohistochemistry-paraffin (IHC-P): 1/200-1/400

    Target

    • Alternative Names
    • Nucleolin; Protein C23; Nsr1; C23;
    • Long Name
    • Nucleolin
    • Cellular Localization
    • Cytoplasm; Nucleolus

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "NCL"

    Select a product category from the dropdown menu below to view related products.
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    IgG Antibody Products Fab Antibody Products ScFv Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOB-693 Recombinant Anti-human NCL Antibody ELISA, IF, FuncS IgG
    MOB-1491CT Recombinant Mouse anti-Human NCL Monoclonal antibody (240DU49.2.6) IHC-P, WB
    MRO-2089-CN Rabbit Anti-NCL Polyclonal Antibody (MRO-2089-CN) WB, IF Rabbit IgG
    MRO-2090-CN Rabbit Anti-NCL Polyclonal Antibody (MRO-2090-CN) WB, IF, IHC Rabbit IgG
    VS3-WK2077 Rabbit Anti-NCL (pT76) Recombinant Antibody (clone R74-1C-4) WB, IHC-P Rabbit IgG

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