Rabbit Anti-NCL Recombinant Antibody (VS13-YC770)
CAT#: VS13-YC770
The Rabbit Anti-NCL Recombinant Antibody is specific for human, mouse and rat NCL. NCL encodes a nucleolar phosphoprotein, integral to ribosomal synthesis, spanning 14 exons, with small nucleolar RNA encoded within an intron. The anti-NCL antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/200), Immunohistochemistry-paraffin (1/200-1/400).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71110/1925_C12_8_cr5ed0fca081eb8_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/669_C1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/ihc_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_B_7_5.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_9_4.jpg
Normal Tissue
Figure 8 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4210/19179_A_2_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23981/53440_A_7_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115053-NCL
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Specifications
- Immunogen
- Recombinant human CSDE1, aa 661-710.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NCL
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/200
Immunohistochemistry-paraffin (IHC-P): 1/200-1/400
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NCL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-693-F(E) | Recombinant Anti-human NCL Antibody Fab Fragment | IP, Neut, FuncS | Fab |
| MHH-693-F(E) | Recombinant Human Anti-human NCL Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| HPAB-M0579-YC-F(E) | Human Anti-NCL Recombinant Antibody; Fab Fragment (HPAB-M0579-YC-F(E)) | ELISA, WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-693-S(P) | Recombinant Anti-human NCL Antibody scFv Fragment | WB, FC, FuncS | scFv |
| MHH-693-S(P) | Recombinant Human Anti-human NCL Antibody scFv Fragment | WB, IP, FuncS | scFv |
| HPAB-M0579-YC-S(P) | Human Anti-NCL Recombinant Antibody; scFv Fragment (HPAB-M0579-YC-S(P)) | ELISA, WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1491CT | Recombinant Mouse anti-Human NCL Monoclonal antibody (240DU49.2.6) | IHC-P, WB | |
| MRO-2089-CN | Rabbit Anti-NCL Polyclonal Antibody (MRO-2089-CN) | WB, IF | Rabbit IgG |
| MRO-2090-CN | Rabbit Anti-NCL Polyclonal Antibody (MRO-2090-CN) | WB, IF, IHC | Rabbit IgG |
| HPAB-M0579-YC | Human Anti-NCL Recombinant Antibody (HPAB-M0579-YC) | ELISA, WB, FuncS | Human IgG, κ |
| VS3-WK2005 | Rabbit Anti-NCL (pT84) Recombinant Antibody (clone R06-4D0) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2381 | Hi-Affi™ Recombinant Rabbit Anti-NCL Monoclonal Antibody (DS2381AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1463 | Anti-NCL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4713 | Anti-Human NCL Immunohistochemistry Kit | IHC |
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