Anti-NCSTN Antibody Prodrug, Protease Activated (A5201A)
CAT#: VS-1025-YC103
The Anti-NCSTN Antibody Prodrug, Protease Activated (A5201A) is an antibody prodrug comprised of a recombinant human antibody targeting human NCT, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to NCSTN protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to NCSTN effectively. NCT (Nicastrin) is an essential component of the gamma-secretase complex, which cleaves the NOTCH receptor to release its active intracellular domain. By facilitating the release of the NOTCH transcription factor, Nicastrin indirectly contributes to the oncogenic signaling of NOTCH in T-ALL. Inhibitors of gamma-secretase are designed to block this cleavage and signaling.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70642/1351_B11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54846/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21982/151332_A_5_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54846/139520_B_9_5.jpg
Normal Tissue
Figure 5 Cerebellum
Purkinje cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54846/139520_B_9_8.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54846/139520_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21982/151332_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54846/139520_A_7_5.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:
Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21982/151332_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162736-NCSTN
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human NCT
- Target
- NCSTN
- Species Reactivity
- Human
- Clone
- A5201A
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimers disease; however, the nature of the encoded proteins role in Alzheimers disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
- Alternative Names
- NCSTN; nicastrin; ATAG1874; anterior pharynx-defective 2
- Gene ID
- 23385
- UniProt ID
- Q92542
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Datasheet
MSDS
COA
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-694 | Recombinant Anti-human NCSTN Antibody | ELISA, IP, FuncS | IgG |
| MHH-694 | Recombinant Human Anti-human NCSTN Antibody | RIA, IF, FuncS | IgG |
| MOB-2454CT | Recombinant Mouse anti-Human NCSTN Monoclonal antibody (nBcdbn) | WB, IHC-P | |
| MRO-1095-CN | Recombinant Rabbit Anti-NCSTN Monoclonal Antibody (JG68-33) | WB | Rabbit IgG |
| VS3-FY1012 | Recombinant Rabbit Anti-NCSTN Antibody (clone R02-9E6) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-694-F(E) | Recombinant Anti-human NCSTN Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
| MHH-694-F(E) | Recombinant Human Anti-human NCSTN Antibody Fab Fragment | ELISA, WB, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-694-S(P) | Recombinant Anti-human NCSTN Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| MHH-694-S(P) | Recombinant Human Anti-human NCSTN Antibody scFv Fragment | WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-479MZ | Anti-Human NCSTN Recombinant Antibody (A5201A) | ELISA, FuncS, ICC | Human antibody |
| TAB-479MZ-S(P) | Anti-Human NCSTN Recombinant Antibody scFv Fragment (A5201A) | IS | Human antibody |
| TAB-479MZ-F(E) | Anti-Human NCSTN Recombinant Antibody Fab Fragment (A5201A) | IS | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2386 | Hi-Affi™ Recombinant Rabbit Anti-NCSTN Monoclonal Antibody (DS2386AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1086 | AbPlus™ Anti-NCSTN Magnetic Beads (VS-0724-YC1086) | IP, Protein Purification | |
| VS-0724-YC1160 | AbPlus™ Anti-Ncstn Magnetic Beads (VS-0724-YC1160) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC138 | Recombinant Anti-NCSTN Vesicular Antibody, EV Displayed (VS-0425-YC138) | ELISA, FC, Cell-uptake |
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