Rabbit Anti-NDRG1 (pS330) Recombinant Antibody (clone R05-1D2)
CAT#: VS3-WK2035
This product is a rabbit recombinant antibody that is specific to phosphorylated NDRG1. The target of this antibody is phospho-NDRG1 (Ser330). The clone R05-1D2 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of NDRG1 (pSer330) observed in WB experiments using this antibody is 43 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/18545_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/18545_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/18545_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/18545_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6881/18545_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTCEpi, hTERT-HME1, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104419-NDRG1
❮
❯
ICC
Figure 1 Rabbit Anti-NDRG1 (pSer330) Recombinant Antibody (clone R05-1D2) in ICC.
Immunocytochemistry analysis of phospho-NDRG1 (Ser330) (green) in 293T using anti-NDRG1 (pSer330) antibody, Cat# VS3-WK2035 and DAPI (blue).
❮
❯
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser330 of human NDRG1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser330 of human and mouse NDRG1
- Species Reactivity
- Human, Mouse
- Clone
- R05-1D2
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- N-Myc Downstream Regulated 1; Reducing Agents And Tunicamycin-Responsive Protein; N-Myc Downstream-Regulated Gene 1 Protein; Nickel-Specific Induction Protein Cap43; Differentiation-Related Gene 1 Protein; DRG-1; RIT42; CAP43; DRG1; Rtp; Protein Regulated By Oxygen-1
- Gene ID
- 10397
- UniProt ID
- Q92597
- Long Name
- N-Myc Downstream Regulated 1
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK2035. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "NDRG1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1312MZ | Recombinant Mouse Anti-Human NDRG1 Antibody | WB | Mouse antibody |
| MRO-1066-CN | Recombinant Rabbit Anti-NDRG1 Monoclonal Antibody (JM32-02) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0482U | Rabbit Anti-NDRG1 Recombinant Antibody (clone 4C8) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS13-YC776 | Rabbit Anti-NDRG1 Recombinant Antibody (VS13-YC776) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0220MZ | Chicken Anti-GC4 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0221MZ | Chicken Anti-GC4 (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0973MZ | Chicken Anti-NDRG1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2389 | Hi-Affi™ Recombinant Rabbit Anti-NDRG1 Monoclonal Antibody (DS2389AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1240 | AbPlus™ Anti-NDRG1 Magnetic Beads (VS-0724-YC1240) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4725 | Anti-NDRG1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, ELISA, FuncS
Application: ELISA
Application: WB, ELISA, IF
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IF, Block, FuncS
-2.png)
Application: ELISA
Application: ELISA, Block, WB, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
