Rabbit Anti-NDRG1 Recombinant Antibody (clone 4C8) (CAT#: ZG-0482U)
Rabbit Anti-NDRG1 Antibody (clone 4C8) displayed high specificity against the NDRG1 protein of Human and Mouse.
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Custom Production
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WB
Figure 1 Rabbit Anti-NDRG1 Antibody (ZG-0482U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissue
All lanes: NDRG1 antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 36, 34 KDa
Observed band size: 43 KDa
IHC
Figure 2 Rabbit Anti-NDRG1 Antibody (ZG-0482U) in IHC
IHC image of ZG-0482U diluted at 1:71.67 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-NDRG1 Antibody (ZG-0482U) in IF
Immunofluorescence staining of Hela cells with ZG-0482U at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human NDRG1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDRG1
- Species Reactivity
- Human, Mouse
- Clone
- 4C8
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Charcot-Marie-Tooth Disease, Type 4D and Neuropathy, Congenital Hypomyelinating, 1, Autosomal Recessive.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Protein NDRG1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42; NDRG1; CAP43; DRG1; RTP
- Gene ID
- 10397
- UniProt ID
- Q92597
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| VS3-WK2035 | Rabbit Anti-NDRG1 (pS330) Recombinant Antibody (clone R05-1D2) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS13-YC776 | Rabbit Anti-NDRG1 Recombinant Antibody (VS13-YC776) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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