Rabbit Anti-NDUFAF1 Recombinant Antibody (clone R04-2J6)
CAT#: VS3-FY1787
This R04-2J6 recombinant monoclonal antibody has been genetically engineered to specifically target human NDUFAF1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the NDUFAF1 antibody was determined using western blot analysis of NDUFAF1 in K562, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-NDUFAF1 Antibody (VS3-FY1787) in WB.
The NDUFAF1 expression in K562, Hela lysates were detected using the anti-NDUFAF1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/565_B11_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137806-NDUFAF1
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Specifications
- Immunogen
- Synthetic peptide of human NDUFAF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDUFAF1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-2J6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 35 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 11 and Isolated Complex I Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; CIA30; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; CGI-65; CGI65
- Gene ID
- 51103
- UniProt ID
- Q9Y375
- Sequence Similarities
- Belongs to the CIA30 family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y375)
Modification sites at neXtProt (NX_Q9Y375)
- Protein Refseq
- XP_006720618.1; XP_011519960.1; XP_011519961.1; XP_024305713.1; XP_047288593.1
- Function
- As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NDUFAF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2399 | Hi-Affi™ Recombinant Rabbit Anti-NDUFAF1 Monoclonal Antibody (DS2399AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK373 | Rabbit Anti-NDUFAF1 Recombinant Antibody (clone JE47-18) | WB, ICC, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1471 | Anti-NDUFAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4736 | Anti-Human NDUFAF1 Immunohistochemistry Kit | IHC |
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