Rabbit Anti-NDUFAF1 Recombinant Antibody (clone R04-2J6) (CAT#: VS3-FY1787)
This R04-2J6 recombinant monoclonal antibody has been genetically engineered to specifically target human NDUFAF1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the NDUFAF1 antibody was determined using western blot analysis of NDUFAF1 in K562, Hela lysates.
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WB
Figure 1 Anti-NDUFAF1 Antibody (VS3-FY1787) in WB.
The NDUFAF1 expression in K562, Hela lysates were detected using the anti-NDUFAF1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NDUFAF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDUFAF1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-2J6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 35 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 11 and Isolated Complex I Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; CIA30; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; CGI-65; CGI65
- Gene ID
- 51103
- UniProt ID
- Q9Y375
- Sequence Similarities
- Belongs to the CIA30 family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y375)
Modification sites at neXtProt (NX_Q9Y375)
- Protein Refseq
- XP_006720618.1; XP_011519960.1; XP_011519961.1; XP_024305713.1; XP_047288593.1
- Function
- As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2399 | Hi-Affi™ Recombinant Rabbit Anti-NDUFAF1 Monoclonal Antibody (DS2399AB) | FC, ICC, IHC-P, IP, WB | IgG |
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