Rabbit Anti-NDUFS3 Recombinant Antibody (clone 1H12) (CAT#: ZG-0483U)
Rabbit Anti-NDUFS3 Antibody (clone 1H12) displayed high specificity against the NDUFS3 protein of Human.
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WB
Figure 1 Rabbit Anti-NDUFS3 Antibody (ZG-0483U) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate,
All lanes: NDUFS3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 15 KDa
Observed band size: 31 kDa
IHC
Figure 2 Rabbit Anti-NDUFS3 Antibody (ZG-0483U) in IHC
IHC image of ZG-0483U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human NDUFS3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDUFS3
- Species Reactivity
- Human
- Clone
- 1H12
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 8 and Mitochondrial Complex I Deficiency, Nuclear Type 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; mitochondrial; EC 1.6.5.3; EC 1.6.99.3; Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit; NDUFS3
- Gene ID
- 4277
- UniProt ID
- O75489
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3179z | Mouse Anti-NDUFS3 Recombinant Antibody (clone 2F10) | WB, ELISA, IHC, IP | Mouse IgG2b, κ |
| MRO-1068-CN | Recombinant Rabbit Anti-NDUFS3 Monoclonal Antibody (JU10-32) | WB, IHC, IF, IP | Rabbit IgG |
| MRO-2062-CN | Rabbit Anti-NDUFS3 Polyclonal Antibody (MRO-2062-CN) | WB, IHC, FC | Rabbit IgG |
| VS3-WK631 | Rabbit Anti-NDUFS3 Recombinant Antibody (clone JU10-32) | WB, ELISA | Rabbit IgG |
| VS3-FY1019 | Recombinant Rabbit Anti-NDUFS3 Antibody (clone R06-4H2) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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