Rabbit Anti-NDUFS3 Recombinant Antibody (clone 1H12) (CAT#: ZG-0483U)
Rabbit Anti-NDUFS3 Antibody (clone 1H12) displayed high specificity against the NDUFS3 protein of Human.
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WB
Figure 1 Rabbit Anti-NDUFS3 Antibody (ZG-0483U) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate,
All lanes: NDUFS3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 15 KDa
Observed band size: 31 kDa
IHC
Figure 2 Rabbit Anti-NDUFS3 Antibody (ZG-0483U) in IHC
IHC image of ZG-0483U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human NDUFS3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDUFS3
- Species Reactivity
- Human
- Clone
- 1H12
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 8 and Mitochondrial Complex I Deficiency, Nuclear Type 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; mitochondrial; EC 1.6.5.3; EC 1.6.99.3; Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit; NDUFS3
- Gene ID
- 4277
- UniProt ID
- O75489
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3179z | Mouse Anti-NDUFS3 Recombinant Antibody (clone 2F10) | WB, ELISA, IHC, IP | Mouse IgG2b, κ |
| MRO-1068-CN | Recombinant Rabbit Anti-NDUFS3 Monoclonal Antibody (JU10-32) | WB, IHC, IF, IP | Rabbit IgG |
| MRO-2062-CN | Rabbit Anti-NDUFS3 Polyclonal Antibody (MRO-2062-CN) | WB, IHC, FC | Rabbit IgG |
| VS3-WK631 | Rabbit Anti-NDUFS3 Recombinant Antibody (clone JU10-32) | WB, ELISA | Rabbit IgG |
| VS3-FY1019 | Recombinant Rabbit Anti-NDUFS3 Antibody (clone R06-4H2) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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