Anti-NEDD8 Immunohistochemistry Kit (CAT#: VS-0325-XY1477)
Equipped with all essential reagents, the NEDD8 IHC Kit ensures a seamless experimental experience. The optimized protocol streamlines procedures, saving time and reducing errors. Compatible with paraffin - and frozen - section samples, it's a reliable choice for studying NEDD8.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27583/1762_H7_3_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27583/1762_H7_3_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60254/1123_C6_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60254/1123_C6_2_selected.jpg

Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/624_G8_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/624_G8_1_selected.jpg

Figure 4 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/ihc_selected.jpg

Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_B_8_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_B_8_5.jpg

Figure 6 Parathyroid gland
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_3_8.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_3_8.jpg

Figure 7 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_3.jpg

Figure 8 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_8_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_8_5.jpg

Figure 9 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_5_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_5_6.jpg

Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_8.jpg

Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129559-NEDD8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129559-NEDD8
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- NEDD8
- Primary Antibody
- Rabbit Anti-NEDD8 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Single Domain Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-945-VHH | Recombinant Anti-human NEDD8 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
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