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    Hi-Affi™ Recombinant Rabbit Anti-NEDD8 Monoclonal Antibody (DS2409AB) (CAT#: MOR-2409)

    Recombinant Rabbit anti-NEDD8 monoclonal antibody for ICC/IF, IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line Hep G2
    Figure 1 IF staining of human cell line Hep G2
    (Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27583/1762_H7_3_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60254/1123_C6_2_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/624_G8_1_selected.jpg
    Figure 4 IHC staining of human cerebral cortex
    Figure 4 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/ihc_selected.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_B_8_5.jpg
    Figure 6 Parathyroid gland
    Figure 6 Parathyroid gland
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_3_8.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_3.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_8_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_5_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129559-NEDD8

    Specifications

    • Immunogen
    • Synthetic peptide corresponding to residues in the N terminal of human NEDD8.
    • Host Species
    • Rabbit
    • Derivation
    • Phage display library screening
    • Type
    • IgG
    • Species Reactivity
    • Human
    • Clone
    • DS2409AB
    • Applications
    • Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Paraffin); Immunoprecipitation; Western Blot
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
    • Format
    • Liquid
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Target

    • Alternative Names
    • NEDD-8

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "NEDD8"

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    Single Domain Antibody Products IgG Antibody Products Non-Human Primate (NHP) Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    NAB-945-VHH Recombinant Anti-human NEDD8 VHH Single Domain Antibody IHC, ICC, IP, ChiP, Neut Llama VHH

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