Rabbit Anti-NHP NEDD8 Recombinant Antibody (clone B.226.2)
CAT#: VS-1024-XY338
This antibody, directed against NEDD8, exhibits impressive reactivity with human, mouse, non-human primate, and rat samples. Generated from a synthetic peptide of NEDD8, it is engineered for a wide range of applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), and Immunoprecipitation (IP). This rabbit-derived antibody is an essential asset for researchers aiming to explore the intricacies of protein modification and its implications in cellular signaling. Utilize this tool to enhance your investigations into ubiquitin-like pathways and their roles in health and disease.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27583/1762_H7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60254/1123_C6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/624_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_B_8_5.jpg
Normal Tissue
Figure 6 Parathyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_3_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129559-NEDD8
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- NEDD8
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- B.226.2
- Applications
- WB, IHC, ICC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:300, ICC: 1:100, IP: 1:25
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NEDD8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-945-VHH | Recombinant Anti-human NEDD8 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2409 | Hi-Affi™ Recombinant Rabbit Anti-NEDD8 Monoclonal Antibody (DS2409AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1072-CN | Recombinant Rabbit Anti-NEDD8 Monoclonal Antibody (JF69-09) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2063-CN | Rabbit Anti-NEDD8 Polyclonal Antibody (MRO-2063-CN) | WB, IF, FC | Rabbit IgG |
| VS3-FY1635 | Rabbit Anti-NEDD8 Recombinant Antibody (clone R02-4G8) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| VS13-YC781 | Rabbit Anti-NEDD8 Recombinant Antibody (VS13-YC781) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR460 | Mouse Anti-NEDD8 Recombinant Antibody (clone 5B8) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1477 | Anti-NEDD8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4769 | Anti-Human NEDD8 Immunohistochemistry Kit | IHC |
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