Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker (CAT#: VS-0924-YC75)
This product is an anti-NES recombinant antibody (VS-0924-YC75) designed to target the human NES protein, a marker for cancer stem cells (CSCs). It can be used for detecting NES in CSC tissues or cells using various methods: Immunohistochemistry (1/1000-1/2500), Immunofluorescence (2-10 µg/mL), and Western blot (1 µg/mL).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Figure 2 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Figure 4 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NES
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/2500-1/5000
Western Blot (WB): 1 µg/mL
Immunofluorescence (IF): 2-10 µg/mL
Target
- Alternative Names
- FLJ21841
- Gene ID
- 10763
- UniProt ID
- P48681
- Long Name
- Nestin
- Cellular Localization
- #Intermediate filament
- Post Translation Modifications
- #Acetylation
#Isopeptide bond
#Phosphoprotein
#Ubl conjugation
- Function
- #Developmental protein
Recommended Products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1670z | Mouse Anti-NES Recombinant Antibody (clone 28C6) | WB, FC, ICC, IHC, IP | Mouse IgG1 |
| MOB-1043MZ | Recombinant Mouse Anti-Human NES Antibody (clone 3D2.4B22) | FC, IP WB | Mouse antibody |
| MRO-1075-CN | Recombinant Rabbit Anti-NES Monoclonal Antibody (CBACN-397) | IF, IHC | Rabbit IgG |
| MRO-2064-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) | IHC | Rabbit IgG |
| MRO-2065-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2065-CN) | IF, IHC, FC, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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