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    Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker (CAT#: VS-0924-YC75)

    This product is an anti-NES recombinant antibody (VS-0924-YC75) designed to target the human NES protein, a marker for cancer stem cells (CSCs). It can be used for detecting NES in CSC tissues or cells using various methods: Immunohistochemistry (1/1000-1/2500), Immunofluorescence (2-10 µg/mL), and Western blot (1 µg/mL).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Fc Engineering:

    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
    Figure 2 IF staining of human cell line AF22
    Figure 2 IF staining of human cell line AF22
    (Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
    Normal Tissue
    Figure 4 Spleen
    Figure 4 Spleen
    (High expression in spleen)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
    Figure 8 Heart muscle
    Figure 8 Heart muscle
    (Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
    Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES

    Specifications

    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human NES
    • Species Reactivity
    • Human
    • Applications
    • IHC, WB, IF

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Purity
    • >95%
    • Concentration
    • 0.1 mg/mL
    • Buffer
    • PBS, pH 7.2, with 40% glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Immunohistochemistry (IHC): 1/2500-1/5000
      Western Blot (WB): 1 µg/mL
      Immunofluorescence (IF): 2-10 µg/mL

    Target

    • Alternative Names
    • FLJ21841
    • Long Name
    • Nestin
    • Cellular Localization
    • #Intermediate filament
    • Post Translation Modifications
    • #Acetylation
      #Isopeptide bond
      #Phosphoprotein
      #Ubl conjugation
    • Function
    • #Developmental protein

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    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-1043MZ Recombinant Mouse Anti-Human NES Antibody (clone 3D2.4B22) FC, IP WB Mouse antibody
    ZG-0550F Mouse Anti-NES Recombinant Antibody (clone 10C2) WB, IHC, IP Mouse IgG1
    VS3-CJ983 Rabbit Anti-NES Recombinant Antibody (VS3-CJ983) ICC, IF, IHC Rabbit IgG
    VS3-WK1880 Recombinant Rabbit Anti-NES Capture Antibody (clone R65-5M-1) ELISA Rabbit IgG
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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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