Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker
CAT#: VS-0924-YC75
This product is an anti-NES recombinant antibody (VS-0924-YC75) designed to target the human NES protein, a marker for cancer stem cells (CSCs). It can be used for detecting NES in CSC tissues or cells using various methods: Immunohistochemistry (1/1000-1/2500), Immunofluorescence (2-10 µg/mL), and Western blot (1 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NES
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/2500-1/5000
Western Blot (WB): 1 µg/mL
Immunofluorescence (IF): 2-10 µg/mL
Target
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NES"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1670z | Mouse Anti-NES Recombinant Antibody (clone 28C6) | WB, FC, ICC, IHC, IP | Mouse IgG1 |
| MRO-2064-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) | IHC | Rabbit IgG |
| MRO-2065-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2065-CN) | IF, IHC, FC, WB | Rabbit IgG |
| VS1-YJ144 | Mouse Anti-NES Recombinant Antibody (clone 10C2), FITC | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS7-0425-WR464 | Mouse Anti-NES Recombinant Antibody (clone 1A9G12) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0721MZ | Chicken Anti-Nes Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2422 | Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) | IHC-P, WB | IgG |
| MOR-0108-FY | Rabbit Anti-NES Recombinant Antibody (clone AFY0079) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC623 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC623) | IP, Protein Purification | |
| VS-0724-YC624 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC624) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC78 | Rabbit Anti-NES Antibody (VS-0924-YC78) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4792 | Anti-NES Immunohistochemistry Kit | IHC | |
| VS-0525-XY4793 | Anti-Mouse NES Immunohistochemistry Kit | IHC |
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