Rabbit Anti-NES Antibody (VS-0924-YC78) - Cancer Stem Cell Marker
CAT#: VS-0924-YC78
This product is an anti-NES antibody (VS-0924-YC78) designed to target the human NES protein, a marker for cancer stem cells (CSCs). It is suitable for detecting NES in CSC tissues or cells using the following techniques: Immunohistochemistry (1/1000-1/2500), Immunofluorescence (0.5-2 µg/mL), and Western Blot (0.1-0.5 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NES
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/2500-1/5000
Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL
Target
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NES"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0721MZ | Chicken Anti-Nes Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2422 | Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) | IHC-P, WB | IgG |
| MOR-0108-FY | Rabbit Anti-NES Recombinant Antibody (clone AFY0079) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0156U | Mouse Anti-NES Recombinant Antibody (clone 2C2F7) | ELISA, WB, IHC, IF, FC | Mouse IgG1 |
| VS1-YJ144 | Mouse Anti-NES Recombinant Antibody (clone 10C2), FITC | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS1-YJ145 | Mouse Anti-NES Recombinant Antibody (clone 10C2), HRP | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS3-XY1154 | Mouse Anti-NES Recombinant Antibody (clone 3H12B9) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY1155 | Mouse Anti-NES Recombinant Antibody (clone 3H12C8) | ELISA, WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC623 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC623) | IP, Protein Purification | |
| VS-0724-YC624 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC624) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC75 | Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker | IHC, WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4792 | Anti-NES Immunohistochemistry Kit | IHC | |
| VS-0525-XY4793 | Anti-Mouse NES Immunohistochemistry Kit | IHC |
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