Rabbit Anti-NFKBIA Recombinant Antibody (clone 1E9) (CAT#: ZG-0486U)
Rabbit Anti-NFKBIA Antibody (clone 1E9) displayed high specificity against the NFKBIA protein of Human.
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WB
Figure 1 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate
All lanes: NFKBIA antibody at 0.79μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
IHC
Figure 2 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IHC
IHC image of ZG-0486U diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IP
Immunoprecipitating NFKBIA in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0486U in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0486U (3μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human NFKBIA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NFKBIA
- Species Reactivity
- Human
- Clone
- 1E9
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal Dysplasia And Immunodeficiency 2 and Hypohidrotic Ectodermal Dysplasia With Immunodeficiency.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI
- Gene ID
- 4792
- UniProt ID
- P25963
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2435 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) | ICC, IP, WB | IgG |
| MOR-4603 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) | WB, IHC-P | IgG |
| MOR-4604 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH117DS) | WB | IgG |
| MOR-4686 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH200DS) | WB, FC | IgG |
| MOR-0076-FY | Rabbit Anti-NFKBIA Recombinant Antibody (clone AFY0047) | IF, IHC-P, WB | Rabbit IgG |
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