Rabbit Anti-NFKBIA Recombinant Antibody (clone 1E9) (CAT#: ZG-0486U)
Rabbit Anti-NFKBIA Antibody (clone 1E9) displayed high specificity against the NFKBIA protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate
All lanes: NFKBIA antibody at 0.79μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
IHC
Figure 2 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IHC
IHC image of ZG-0486U diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IP
Immunoprecipitating NFKBIA in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0486U in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0486U (3μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human NFKBIA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NFKBIA
- Species Reactivity
- Human
- Clone
- 1E9
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal Dysplasia And Immunodeficiency 2 and Hypohidrotic Ectodermal Dysplasia With Immunodeficiency.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI
- Gene ID
- 4792
- UniProt ID
- P25963
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NFKBIA"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2435 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) | ICC, IP, WB | IgG |
| MOR-4603 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) | WB, IHC-P | IgG |
| MOR-4604 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH117DS) | WB | IgG |
| MOR-4686 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH200DS) | WB, FC | IgG |
| MOR-0076-FY | Rabbit Anti-NFKBIA Recombinant Antibody (clone AFY0047) | IF, IHC-P, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ826 | Mouse Anti-NFKBIA Recombinant Antibody (VS3-CJ826) | WB | Mouse IgG1, κ |
| VS3-CJ827 | Rabbit Anti-NFKBIA (phospho S32) Recombinant Antibody (VS3-CJ827) | WB, ICC, IP | Rabbit IgG |
| VS3-CJ828 | Rabbit Anti-NFKBIA Recombinant Antibody (VS3-CJ828) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY1162 | Mouse Anti-NFKBIA Recombinant Antibody (clone 4D4C4) | IHC, ICC, FC | Mouse IgG1 |
| VS3-XY1163 | Mouse Anti-NFKBIA Recombinant Antibody (clone 2B7) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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