Rabbit Anti-NFKBIA Recombinant Antibody (clone 1E9)
CAT#: ZG-0486U
Rabbit Anti-NFKBIA Antibody (clone 1E9) displayed high specificity against the NFKBIA protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate
All lanes: NFKBIA antibody at 0.79μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
IHC
Figure 2 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IHC
IHC image of ZG-0486U diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-NFKBIA Antibody (ZG-0486U) in IP
Immunoprecipitating NFKBIA in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0486U in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0486U (3μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
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Specifications
- Immunogen
- A synthesized peptide derived from human NFKBIA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NFKBIA
- Species Reactivity
- Human
- Clone
- 1E9
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal Dysplasia And Immunodeficiency 2 and Hypohidrotic Ectodermal Dysplasia With Immunodeficiency.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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