Mouse Anti-NHP ANXA6 Recombinant Antibody (VS-1024-XY26)
CAT#: VS-1024-XY26
This mouse-derived monoclonal antibody targets Annexin A6 (ANXA6) and exhibits broad cross-reactivity with dog, human, mouse, non-human primate, and rat. The immunogen utilized is a recombinant full-length annexin A6, ensuring enhanced specificity and binding affinity. Optimized for use in Western blot (WB) and immunofluorescence (IF) applications, this antibody provides a powerful tool for detecting ANXA6 expression across multiple species, facilitating detailed research into its biological roles and potential implications in cellular functions and diseases.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9977/1914_F7_14_cr5c87753c9ceb1_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2462/9636_B_4_7_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid tissue.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5077/15117_A_1_2_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197043-ANXA6
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Specifications
- Immunogen
- Recombinant full length annexin A6
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ANXA6
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4 with 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, IF: 1:800
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ANXA6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2911z | Mouse Anti-ANXA6 Recombinant Antibody (clone 35H1) | WB, ELISA | Mouse IgG1 |
| ZG-0290F | Mouse Anti-ANXA6 Recombinant Antibody (ZG-0290F) | WB | Mouse IgG |
| VS3-QX55 | Mouse Anti-ANXA6 Recombinant Antibody (clone 8A7-F8-G12) | WB | Mouse IgG1 |
| VS4-WK33 | Mouse Anti-ANXA6 Recombinant Antibody (VS4-WK33) | ELISA, WB | Mouse IgG1 |
| VS3-FY1650 | Rabbit Anti-ANXA6 Recombinant Antibody (clone R07-7G6) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1062 | AbPlus™ Anti-ANXA6 Magnetic Beads (VS-0724-YC1062) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY121 | Anti-ANXA6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY418 | Anti-Human ANXA6 Immunohistochemistry Kit | IHC |
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