Rabbit Anti-NHP CDKN1A Recombinant Antibody (VS-1024-XY358)
CAT#: VS-1024-XY358
This rabbit-derived antibody targets CDKN1A, exhibiting remarkable reactivity with human and non-human primate samples. Generated against a synthetic peptide of CDKN1A, it demonstrates exceptional versatility across multiple applications, including Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FC), and Immunoprecipitation (IP). With its high specificity and sensitivity, this antibody is instrumental in elucidating the role of CDKN1A in cell cycle regulation and tumor suppression. Utilize this powerful tool to deepen your understanding of cellular mechanisms and their implications in disease research.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5946/1541_C12_6_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cervix, uterine
Immunohistochemical staining of human cervix, uterine shows nuclear positivity in squamous cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/156817_B_8_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69401/155899_A_6_5_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_7_3.jpg
Normal Tissue
Figure 5 Pancreas
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_1_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, HSkMC, hTCEpi, LHCN-M2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124762-CDKN1A
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- CDKN1A
- Species Reactivity
- Human, Non-human primate
- Applications
- WB, IHC, FC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:50, FC: 1:100-1:400, IP: 1:50
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDKN1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-190-F(E) | Recombinant Anti-human CDKN1A Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| MHH-190-F(E) | Recombinant Human Anti-human CDKN1A Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-190-S(P) | Recombinant Anti-human CDKN1A Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| MHH-190-S(P) | Recombinant Human Anti-human CDKN1A Antibody scFv Fragment | WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-190 | Recombinant Human Anti-human CDKN1A Antibody | ELISA, IF, FuncS | IgG |
| MOB-2113MZ | Recombinant Mouse Anti-Human CDKN1A Antibody (clone FB20) | FC, ICC, IHC-Fr, IHC-P, IP, WB | Mouse antibody |
| ZG-0797F | Mouse Anti-CDKN1A Recombinant Antibody (clone DCS-60) | WB, IP, IHC | Mouse IgG2a |
| VS3-WK744 | Mouse Anti-CDKN1A Recombinant Antibody (clone Mix-mA) | WB | Mouse IgG1 |
| VS3-CJ925 | Mouse Anti-CDKN1A (C-Terminus) Recombinant Antibody (VS3-CJ925) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0114MZ | Chicken Anti-CDKN1A Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0639 | Hi-Affi™ Rabbit Anti-CDKN1A Recombinant Antibody (clone DS639AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| MOR-4658 | Hi-Affi™ Rabbit Anti-CDKN1A Recombinant Antibody (clone TH172DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0073-FY | Rabbit Anti-CDKN1A Recombinant Antibody (clone AFY0044) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF386 | A*02:01/Human Cyclin-dependent kinase 4 (ALTPVVVTL) MHC Pentamer | FCM | |
| MHC-YF387 | A*02:01/Human Cyclin-dependent kinase inhibitor 1 (FAWERVRGL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY411 | Anti-CDKN1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1332 | Anti-Mouse CDKN1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1331 | Anti-Human CDKN1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1333 | Anti-Monkey CDKN1A Immunohistochemistry Kit | IHC |
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