Mouse Anti-NHP HLA-E Recombinant Antibody (clone MEM-E/02) (CAT#: VS-1024-XY252)
Tailored for the detection of HLA-E, this mouse monoclonal antibody demonstrates reactivity with human and non-human primate tissues. The immunogen is a recombinant human HLA-E denatured heavy chain, ensuring precise targeting. Ideal for immunohistochemistry (IHC), this antibody provides reliable performance for studying HLA-E expression, making it an invaluable tool for in-depth research and diagnostic applications.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31454/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31454/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_B_7_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_B_7_5.jpg
Figure 3 Lung
(Alveolar cells type II
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Macrophages
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_2_4.jpg
(Alveolar cells type II
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Macrophages
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_2_4.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_7_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24589/57001_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204592-HLA-E
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204592-HLA-E
Specifications
- Immunogen
- Recombinant human HLA-E denaturated heavy chain
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HLA-E
- Species Reactivity
- Human, Non-human primate
- Clone
- MEM-E/02
- Applications
- IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 5-10 µg/mL
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-504 | Recombinant Anti-human HLA-E Antibody | ELISA, IF, FuncS | IgG |
| MHH-504 | Recombinant Human Anti-human HLA-E Antibody | ELISA, IF, FuncS | IgG |
| MOB-0943CT | Recombinant Mouse anti-Human HLA-E Monoclonal antibody (NFN-F/03) | IHC-P, WB | |
| FN-168CQ | Mouse Anti-HLA-E Recombinant Antibody (clone 3D12HLA-E) | FC, FuncS, Neut, ELISA | Mouse IgG1, κ |
| VS3-QX535 | Mouse Anti-HLA-E Recombinant Antibody (clone 4G23) | WB, IP, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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