Mouse Anti-NHP RAPGEF1 Recombinant Antibody (clone OTI3H5)
CAT#: VS-1024-XY439
Targeting RAPGEF1, the antibody is generated from a full-length human recombinant protein, ensuring exceptional specificity and reactivity with both human and non-human primate samples. Its robust applications in Western Blotting (WB) and Immunofluorescence (IF) enable detailed exploration of RAPGEF1's role in cellular signaling and morphological changes. With a reputation for precision and reliability, this antibody serves as an essential tool for researchers investigating the molecular mechanisms underlying various physiological and pathological processes, ultimately advancing our understanding of cellular dynamics and disease mechanisms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75984/1715_H7_14_cr57fd30d5be7dd_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6426/17175_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6426/17175_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6426/17175_A_5_6.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6426/17175_B_2_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107263-RAPGEF1
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Specifications
- Immunogen
- Full length human recombinant protein of RAPGEF1
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- RAPGEF1
- Species Reactivity
- Human, Non-human primate
- Clone
- OTI3H5
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:400-1:3000, IF: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAPGEF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1414MZ | Recombinant Mouse Anti-Human RAPGEF1 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5985 | Anti-RAPGEF1 Immunohistochemistry Kit | IHC |
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