Mouse Anti-NHP STUB1 Recombinant Antibody (clone 13I8)
CAT#: VS-1024-XY494
Designed for the precise detection of STUB1, this monoclonal antibody exhibits robust reactivity with human, mouse, non-human primate, and rat samples. Utilizing a human STUB1 recombinant protein as the immunogen, it is optimized for diverse applications, including Western blot, immunohistochemistry, and flow cytometry. Its high specificity and sensitivity facilitate in-depth exploration of STUB1's pivotal roles in protein homeostasis and stress response pathways. Researchers can rely on this antibody to enhance their understanding of the molecular dynamics governing cellular function and disease mechanisms.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43531/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37202/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_B_7_5.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_B_4_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41222/93047_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37202/85463_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103266-STUB1/subcellular
❮
❯
Specifications
- Immunogen
- Human STUB1 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- STUB1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 13I8
- Applications
- WB, IHC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Lyophilized
- Buffer
- PBS with 4 mg trehalose
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.1-0.5 µg/mL, IHC: 0.5-1 µg/mL, FC: 1-3 µg/106 cells
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1534z | Mouse Anti-STUB1 Recombinant Antibody (clone 25B9) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MRO-1449-CN | Recombinant Rabbit Anti-STUB1 Monoclonal Antibody (JG38-22) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK480 | Rabbit Anti-STUB1 Recombinant Antibody (clone JG38-22) | WB, ELISA | Rabbit IgG |
| VS3-WK1219 | Mouse Anti-STUB1 Recombinant Antibody (VS3-WK1219) | WB | Mouse IgG1, κ |
| VS3-FY1410 | Recombinant Rabbit Anti-STUB1 Antibody (clone R05-5F5) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3447 | Hi-Affi™ Recombinant Rabbit Anti-STUB1 Monoclonal Antibody (DS3447AB) | WB, IP, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1340 | AbPlus™ Anti-STUB1 Magnetic Beads (VS-0724-YC1340) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2218 | Anti-STUB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6997 | Anti-Mouse STUB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6996 | Anti-Human STUB1 Immunohistochemistry Kit | IHC |
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