Mouse Anti-NHP UBE2J1 Recombinant Antibody (clone OTI3D1)
CAT#: VS-1024-XY527
Targeting UBE2J1, this antibody is generated from a full-length human recombinant protein, ensuring robust specificity and reactivity across dog, human, mouse, non-human primate, and rat samples. Its diverse applications in Western Blotting (WB), Immunohistochemistry (IHC), and Flow Cytometry (FC) enable researchers to delve into the functional roles of UBE2J1 in ubiquitination and protein turnover. Known for its exceptional sensitivity and reliability, this antibody serves as an essential resource for advancing investigations into the molecular mechanisms underlying various physiological processes and disease conditions, facilitating the pursuit of innovative therapeutic approaches.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of lymphoid cells in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/ihc_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_4_6.jpg
Normal Tissue
Figure 4 High expression in lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_8_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_5_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, Karpas-707, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198833-UBE2J1
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Specifications
- Immunogen
- Full length human recombinant protein of UBE2J1
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- UBE2J1
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI3D1
- Applications
- WB, IHC, FC
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, FC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "UBE2J1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2136CT | Recombinant Mouse anti-Human UBE2J1 Monoclonal antibody (EML1395) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC238 | AbPlus™ Anti-UBE2J1 Magnetic Beads (VS-0724-YC238) | IP, Protein Purification |
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