Mouse Anti-NHP UBE2J1 Recombinant Antibody (clone OTI3D1) (CAT#: VS-1024-XY527)
Targeting UBE2J1, this antibody is generated from a full-length human recombinant protein, ensuring robust specificity and reactivity across dog, human, mouse, non-human primate, and rat samples. Its diverse applications in Western Blotting (WB), Immunohistochemistry (IHC), and Flow Cytometry (FC) enable researchers to delve into the functional roles of UBE2J1 in ubiquitination and protein turnover. Known for its exceptional sensitivity and reliability, this antibody serves as an essential resource for advancing investigations into the molecular mechanisms underlying various physiological processes and disease conditions, facilitating the pursuit of innovative therapeutic approaches.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of lymphoid cells in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of lymphoid cells in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/ihc_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_9_3.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_4_6.jpg
Figure 4 High expression in lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_8_8.jpg
Figure 5 Tonsil
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_5_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73419/153330_A_5_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (Daudi, Karpas-707, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198833-UBE2J1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198833-UBE2J1
Specifications
- Immunogen
- Full length human recombinant protein of UBE2J1
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- UBE2J1
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI3D1
- Applications
- WB, IHC, FC
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, FC: 1:100
Target
- Alternative Names
- UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
- Gene ID
- 51465
- UniProt ID
- Q9Y385
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2136CT | Recombinant Mouse anti-Human UBE2J1 Monoclonal antibody (EML1395) | WB |
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