Mouse Anti-NHP ZSCAN21 Recombinant Antibody (clone OTI2E5) (CAT#: VS-1024-XY550)
Designed to recognize ZSCAN21, this monoclonal antibody exhibits robust reactivity with dog, human, mouse, and non-human primate samples. Employing a full-length human recombinant protein as the immunogen, it is well-suited for diverse applications, including Western blot, immunohistochemistry, and immunofluorescence. Its precise specificity and sensitivity facilitate in-depth exploration of ZSCAN21's role in gene regulation and developmental processes, thereby providing essential insights into its involvement in various biological systems. This antibody represents a critical asset for advancing research in molecular biology and related fields.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in tubular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in tubular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/144_F7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/144_F7_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/143_F7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/143_F7_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_9_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_5.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000166529-ZSCAN21
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000166529-ZSCAN21
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166529-ZSCAN21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166529-ZSCAN21
Specifications
- Immunogen
- Full length human recombinant protein of ZSCAN21
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ZSCAN21
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI2E5
- Applications
- WB, IHC, IF
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200-1:3000, IHC: 1:200, IF: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-850 | Human Anti-ZSCAN21 Recombinant Antibody (clone T226) | IP, ELISA | Human IgG |
| MOB-2244CT | Recombinant Mouse anti-Human ZSCAN21 Monoclonal antibody (EML739) | WB |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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