Mouse Anti-NHP ZSCAN21 Recombinant Antibody (clone OTI2E5)
CAT#: VS-1024-XY550
Designed to recognize ZSCAN21, this monoclonal antibody exhibits robust reactivity with dog, human, mouse, and non-human primate samples. Employing a full-length human recombinant protein as the immunogen, it is well-suited for diverse applications, including Western blot, immunohistochemistry, and immunofluorescence. Its precise specificity and sensitivity facilitate in-depth exploration of ZSCAN21's role in gene regulation and developmental processes, thereby providing essential insights into its involvement in various biological systems. This antibody represents a critical asset for advancing research in molecular biology and related fields.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in tubular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/144_F7_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16948/143_F7_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23591/52319_A_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166529-ZSCAN21
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Specifications
- Immunogen
- Full length human recombinant protein of ZSCAN21
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ZSCAN21
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI2E5
- Applications
- WB, IHC, IF
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200-1:3000, IHC: 1:200, IF: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ZSCAN21"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-850 | Human Anti-ZSCAN21 Recombinant Antibody (clone T226) | IP, ELISA | Human IgG |
| MOB-2244CT | Recombinant Mouse anti-Human ZSCAN21 Monoclonal antibody (EML739) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY8073 | Anti-ZSCAN21 Immunohistochemistry Kit | IHC | |
| VS-0525-XY8074 | Anti-Mouse ZSCAN21 Immunohistochemistry Kit | IHC |
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