CytoStream™ Rabbit Anti-NLK Recombinant Antibody (VS13-YC815)
CAT#: VS13-YC815
The CytoStream™ Rabbit Anti-NLK Recombinant Antibody is specific for human NLK. NLK is a serine/threonine kinase affecting transcription factors in cell fate, signaling via pathways like Wnt, and influencing transcriptional activity and cellular pathway dynamics. The anti-NLK antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18192/150_A6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_B_7_5_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087095-NLK
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Specifications
- Immunogen
- Recombinant human NLK.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NLK
- Species Reactivity
- Human
- Applications
- WB, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Flow cytometry (FC): 1/50-1/100
Target
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NLK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1728MZ | Recombinant Mouse Anti-Human NLK Antibody | WB | Mouse antibody |
| VS3-QX820 | Mouse Anti-NLK Recombinant Antibody (clone 1146CT24.2.1) | WB | Mouse IgG2a |
| VS3-WK1184 | Mouse Anti-NLK Recombinant Antibody (VS3-WK1184) | WB | Mouse IgG2a |
| VS3-FY2820 | Mouse Anti-NLK Recombinant Antibody (VS3-FY2820) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2450 | Hi-Affi™ Recombinant Rabbit Anti-NLK Monoclonal Antibody (DS2450AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4866 | Anti-NLK Immunohistochemistry Kit | IHC |
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