Mouse Anti-NLK Recombinant Antibody (VS3-FY2820) (CAT#: VS3-FY2820)
The anti-NLK antibody is a mouse recombinant monoclonal antibody that is specifically created to bind human NLK protein. It cross-reacts with mouse, rat species. The calculated molecular weight of the protein is about 58.3 kDa. This monoclonal antibody proves valuable in disease research related to Colorectal Cancer and Hepatocellular Carcinoma. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18192/150_A6_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18192/150_A6_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/if_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neuronal and glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_B_7_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neuronal and glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_B_7_5_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_9_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56511/162953_A_9_4.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087095-NLK
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087095-NLK
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat NLK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 58.3 kDa.
- Related Disease
- Colorectal Cancer and Hepatocellular Carcinoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Nemo Like Kinase; Nemo-Like Kinase; EC 2.7.11.24; Protein LAK1; EC 2.7.11; LAK1
- Gene ID
- 51701
- UniProt ID
- Q9UBE8
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Nucleus
Cytoplasm
- Post Translation Modifications
- Phosphorylated on Thr-298.
Intermolecular autophosphorylation on Thr-298 activates the enzyme.
(Q9UBE8-NLK_HUMAN)
Modification sites at PhosphoSitePlus (Q9UBE8)
Modification sites at neXtProt (NX_Q9UBE8)
- Protein Refseq
- XP_054172389.1; NP_057315.3
- Function
- Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed:15004007, 15764709).
Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613).
Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1728MZ | Recombinant Mouse Anti-Human NLK Antibody | WB | Mouse antibody |
| VS3-QX820 | Mouse Anti-NLK Recombinant Antibody (clone 1146CT24.2.1) | WB | Mouse IgG2a |
| VS3-WK1184 | Mouse Anti-NLK Recombinant Antibody (VS3-WK1184) | WB | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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