Rabbit Anti-NME2 Recombinant Antibody (clone R08-1C1)
CAT#: VS3-FY2050
The R08-1C1 recombinant monoclonal antibody is specifically designed to target human NME2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. This NME2 antibody has been teted positive by western blot analysis of NME2 in mouse spleen lysates and western blot analysis of NME2 in U2OS, C6, Ramos lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & midbody ring.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36213/1894_H6_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36214/1894_A7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243678-NME2
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WB
Figure 1 Anti-NME2 Antibody (VS3-FY2050) in WB.
The NME2 expression in mouse spleen lysates were detected using the anti-NME2 by western blot assay.
WB
Figure 2 Anti-NME2 Antibody (VS3-FY2050) in WB.
The NME2 expression in U2OS, C6, Ramos lysates were detected using the anti-NME2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NME2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NME2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-1C1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 17 kDa.
- Related Disease
- Endometrium Carcinoma In Situ and Nemaline Myopathy 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 2; Non-Metastatic Cells 2, Protein (NM23B) Expressed In; C-Myc Purine-Binding Transcription Factor PUF; Histidine Protein Kinase NDKB; NDP Kinase B; EC 2.7.4.6; NM23-H2; NM23B; Epididymis Secretory Sperm Binding Protein Li 155an; Non-Metastatic Cells 2, Protein (NM23) Expressed In; Nucleoside Diphosphate Kinase B
- Gene ID
- 4831
- UniProt ID
- P22392
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cell projection, Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys39 and Lys128 (NX_P22392 [NX_P22392-1])
Modification sites at PhosphoSitePlus (P22392)
Glycosylation from GlyGen (P22392) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001018147.1; NP_001018148.1; NP_001018149.1; NP_001185611.1; NP_002503.1
- Function
- Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically.
Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III1 region of the MYC gene promoter. Does not bind to duplex NHE III1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NME2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3844z | Mouse Anti-NME2 Recombinant Antibody (clone 42H8) | WB, IP, ELISA | Mouse IgG1, κ |
| VS3-XY1169 | Mouse Anti-NME2 Recombinant Antibody (clone 2F3E6) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-XY1170 | Mouse Anti-NME2 Recombinant Antibody (clone 6B4B5) | ELISA, WB, IHC | Mouse IgG1 |
| VS7-0425-WR487 | Mouse Anti-NME2 Recombinant Antibody (clone 4G7A8) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2457 | Hi-Affi™ Recombinant Rabbit Anti-NME2 Monoclonal Antibody (DS2457AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC819 | CytoStream™ Rabbit Anti-NME2 Recombinant Antibody (VS13-YC819) | WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4875 | Anti-NME2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4876 | Anti-Mouse NME2 Immunohistochemistry Kit | IHC |
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