CytoStream™ Rabbit Anti-NME2 Recombinant Antibody (VS13-YC819)
CAT#: VS13-YC819
The CytoStream™ Rabbit Anti-NME2 Recombinant Antibody is specific for human, mouse and rat NME2. NME2, alongside NME1, forms a nucleoside diphosphate kinase hexamer, regulating nucleotide synthesis, with read-through transcription producing fusion proteins. The anti-NME2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & midbody ring.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36213/1894_H6_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36214/1894_A7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243678-NME2
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Specifications
- Immunogen
- Recombinant human NME2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NME2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF, FC
- Conjugate
- NONE
- Related Disease
- Endometrium Carcinoma In Situ and Nemaline Myopathy
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/1000
Immunofluorescence (IF): 1/100
Flow cytometry (FC): 1/1000
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 2; Non-Metastatic Cells 2, Protein (NM23B) Expressed In; C-Myc Purine-Binding Transcription Factor PUF; Histidine Protein Kinase NDKB; NDP Kinase B; EC 2.7.4.6; NM23-H2; NM23B; Epididymis Secretory Sperm Binding Protein Li 155an; Non-Metastatic Cells 2, Protein (NM23) Expressed In; Nucleoside Diphosphate Kinase B;
- Gene ID
- 4831
- UniProt ID
- P22392
- Long Name
- NME/NM23 Nucleoside Diphosphate Kinase 2
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cytoplasm; Cell projection; Lamellipodium; Ruffle
- Protein Refseq
- NP_001018147.1; NP_001018148.1; NP_001018149.1; NP_001185611.1; NP_002503.1
- Function
- Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically.
Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III1 region of the MYC gene promoter. Does not bind to duplex NHE III1.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NME2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3844z | Mouse Anti-NME2 Recombinant Antibody (clone 42H8) | WB, IP, ELISA | Mouse IgG1, κ |
| VS3-XY1169 | Mouse Anti-NME2 Recombinant Antibody (clone 2F3E6) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-XY1170 | Mouse Anti-NME2 Recombinant Antibody (clone 6B4B5) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY2050 | Rabbit Anti-NME2 Recombinant Antibody (clone R08-1C1) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR487 | Mouse Anti-NME2 Recombinant Antibody (clone 4G7A8) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2457 | Hi-Affi™ Recombinant Rabbit Anti-NME2 Monoclonal Antibody (DS2457AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4875 | Anti-NME2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4876 | Anti-Mouse NME2 Immunohistochemistry Kit | IHC |
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