Mouse Anti-NME2 Recombinant Antibody (clone 4G7A8)
CAT#: VS7-0425-WR487
This product is a mouse IgG1 antibody that binds to human NME2. The antibody 4G7A8 recognizes specific amino acid domain of human NME2 (AA: FULL(1-152)). It can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & midbody ring.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36213/1894_H6_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36214/1894_A7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243678-NME2
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Specifications
- Immunogen
- Recombinant human NME2 (AA: FULL(1-152)) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NME2
- Species Reactivity
- Human
- Clone
- 4G7A8
- Molecule Class
- Nucleoside Diphosphate Kinases
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NME2; Non-Metastatic Cells 2, Protein (NM23B) Expressed In; NDP Kinase B; HEL-S-155an; Non-Metastatic Cells 2, Protein (NM23) Expressed In; C-Myc Transcription Factor; NDK B; NME/NM23 Nucleoside Diphosphate Kinase 2; EC 2.7.13.3; Histidine Protein Kinase
- Gene ID
- 4831
- UniProt ID
- P22392
- Long Name
- NME/NM23 Nucleoside Diphosphate Kinase 2
- Cellular Localization
- Cell projection; Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001018147.1
- Function
- Activator; DNA-binding; Kinase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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