Rabbit Anti-NME3 Recombinant Antibody (clone R03-3H6) (CAT#: VS3-FY2051)
The western blot analysis of NME3 in A549, HL-60 lysates using this rabbit recombinant monoclonal antibody, which demonstrates binding to human NME3. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R03-3H6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Specific Inquiry
WB
Figure 1 Anti-NME3 Antibody (VS3-FY2051) in WB.
The NME3 expression in A549, HL-60 lysates were detected using the anti-NME3 by western blot assay.
IHC
Figure 2 Anti-NME3 Antibody (VS3-FY2051) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-NME3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human NME3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NME3
- Species Reactivity
- Human
- Clone
- R03-3H6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 19 kDa, while the observed molecular weight stands at 19 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 3; Non-Metastatic Cells 3, Protein Expressed In; Nucleoside Diphosphate Kinase C; NDP Kinase 3; EC 2.7.4.6; NM23-H3; DR-Nm23; NDK 3
- Gene ID
- 4832
- UniProt ID
- Q13232
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- Ubiquitination at Lys29 and Lys51 (NX_Q13232 [NX_Q13232-1])
Modification sites at PhosphoSitePlus (Q13232)
- Protein Refseq
- XP_005255389.1; XP_011520805.1; XP_011520806.1; XP_054236378.1; XP_054236379.1
- Function
- The ATP gamma phosphate is transferred to the nucleoside diphosphate beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis.
Inhibits granulocyte differentiation.
May be required for ciliary function during renal development (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NME3"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2458 | Hi-Affi™ Recombinant Rabbit Anti-NME3 Monoclonal Antibody (DS2458AB) | WB, IHC-P, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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