Rabbit Anti-NME3 Recombinant Antibody (clone R03-3H6)
CAT#: VS3-FY2051
The western blot analysis of NME3 in A549, HL-60 lysates using this rabbit recombinant monoclonal antibody, which demonstrates binding to human NME3. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R03-3H6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Tested Data
Gene Expression
WB
Figure 1 Anti-NME3 Antibody (VS3-FY2051) in WB.
The NME3 expression in A549, HL-60 lysates were detected using the anti-NME3 by western blot assay.
IHC
Figure 2 Anti-NME3 Antibody (VS3-FY2051) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-NME3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52733/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103024-NME3
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Specifications
- Immunogen
- Synthetic peptide of human NME3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NME3
- Species Reactivity
- Human
- Clone
- R03-3H6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 19 kDa, while the observed molecular weight stands at 19 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 3; Non-Metastatic Cells 3, Protein Expressed In; Nucleoside Diphosphate Kinase C; NDP Kinase 3; EC 2.7.4.6; NM23-H3; DR-Nm23; NDK 3
- Gene ID
- 4832
- UniProt ID
- Q13232
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- Ubiquitination at Lys29 and Lys51 (NX_Q13232 [NX_Q13232-1])
Modification sites at PhosphoSitePlus (Q13232)
- Protein Refseq
- XP_005255389.1; XP_011520805.1; XP_011520806.1; XP_054236378.1; XP_054236379.1
- Function
- The ATP gamma phosphate is transferred to the nucleoside diphosphate beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis.
Inhibits granulocyte differentiation.
May be required for ciliary function during renal development (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NME3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2458 | Hi-Affi™ Recombinant Rabbit Anti-NME3 Monoclonal Antibody (DS2458AB) | WB, IHC-P, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC820 | Rabbit Anti-NME3 Recombinant Antibody (VS13-YC820) | WB, IHC-P, IP | Rabbit IgG |
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