Mouse Anti-NOS1 Recombinant Antibody (clone 2E11G6) (CAT#: VS7-0425-WR488)
This product is a mouse IgG1 antibody that binds to human NOS1. The antibody 2E11G6 recognizes specific amino acid domain of human NOS1 (AA: 17-153). It can be used for ICC and FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
Figure 2 High expression in caudate nucleus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
Figure 4 Kidney
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, RH-30, RPTEC TERT1, U-2 OS)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
Specifications
- Immunogen
- Recombinant human NOS1 (AA: 17-153) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NOS1
- Species Reactivity
- Human
- Clone
- 2E11G6
- Molecule Class
- Nitric Oxide Synthase
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NOS1; NOS Type I; Nitric Oxide Synthase 1 (Neuronal); Nitric Oxide Synthase, Brain; N-NOS; IHPS1; Constitutive NOS; NOS; NC-NOS; Peptidyl-Cysteine S-Nitrosylase NOS1; EC 1.14.13.39
- Gene ID
- 4842
- UniProt ID
- P29475
- Long Name
- Nitric oxide synthase 1
- Cellular Localization
- Cell membrane; Cell projection; Membrane; Synapse
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000611.1
- Function
- Calmodulin-binding; Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2667z | Mouse Anti-NOS1 Recombinant Antibody (clone 20A5) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0790CT | Recombinant Mouse anti-Human NOS1 Monoclonal antibody (OPT-236) | IHC-P | |
| MRO-1100-CN | Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (CBACN-404) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2079-CN | Rabbit Anti-NOS1 Polyclonal Antibody (MRO-2079-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ859 | Rabbit Anti-NOS1 Recombinant Antibody (VS3-CJ859) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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