Mouse Anti-NP (4-hydroxy-3-nitrophenyl acetyl) Recombinant Antibody (clone 3B62) (CAT#: FAMAB-0245CQ)

Figure 1 Typical isothermal titration calorimetric profiles of the interaction between NP-Cap and Fab(3B62) at 30.0 °C.

a, the NP-Cap solution (1.0 mM in 5 mM sodium phosphate buffer, 200 mM sodium chloride, pH 8.0) was injected 20 times in 5-μl increments into 38 μM Fab(3B62) solution, which was dialyzed against the same buffer. Injections were occurred over 10 s at 3-min intervals. b, integrated areas for the above peaks were plotted against the molar ratio (NPCap)/(Fab(3B62)).

Torigoe, H., Nakayama, T., Imazato, M., Shimada, I., Arata, Y., & Sarai, A. (1995). The Affinity Maturation of Anti-4-hydroxy-3-nitrophenylacetyl Mouse Monoclonal Antibody A CALORIMETRIC STUDY OF THE ANTIGEN-ANTIBODY INTERACTION. Journal of Biological Chemistry, 270(38), 22218-22222.

Figure 2 Thermodynamic parameters (∆H (○), ∆Gu (◊), and -T∆Su (∆)) for the associations between NP-Cap and each of Fab(N1G9), Fab(3B44), and Fab(3B62) as a function of temperature.

All experiments were performed in 5 mM sodium phosphate buffer, 200 mM sodium chloride, pH 8.0, at the experimental temperature. The solid lines were obtained from a linear least-squares regression. ∆Cp was calculated from the slope of the regression line of ∆H (○).

Torigoe, H., Nakayama, T., Imazato, M., Shimada, I., Arata, Y., & Sarai, A. (1995). The Affinity Maturation of Anti-4-hydroxy-3-nitrophenylacetyl Mouse Monoclonal Antibody A CALORIMETRIC STUDY OF THE ANTIGEN-ANTIBODY INTERACTION. Journal of Biological Chemistry, 270(38), 22218-22222.

Figure 3 Dependence of the log Ka values of Fab(N1G9) (○), Fab(3B44) (◊), and Fab(3B62) (∆) for NP-Cap upon the logarithm of the sodium chloride concentration at 30 °C.

All experiments were performed in 5 mM sodium phosphate buffer, pH 8.0, containing the experimental concentration of sodium chloride.

Torigoe, H., Nakayama, T., Imazato, M., Shimada, I., Arata, Y., & Sarai, A. (1995). The Affinity Maturation of Anti-4-hydroxy-3-nitrophenylacetyl Mouse Monoclonal Antibody A CALORIMETRIC STUDY OF THE ANTIGEN-ANTIBODY INTERACTION. Journal of Biological Chemistry, 270(38), 22218-22222.

Figure 4 Correlations between the log Ka values at 25 °C and each absolute value of ∆H at 25 °C (a), ∆Cp (b), and ∆Su at 25 °C(c).

Torigoe, H., Nakayama, T., Imazato, M., Shimada, I., Arata, Y., & Sarai, A. (1995). The Affinity Maturation of Anti-4-hydroxy-3-nitrophenylacetyl Mouse Monoclonal Antibody A CALORIMETRIC STUDY OF THE ANTIGEN-ANTIBODY INTERACTION. Journal of Biological Chemistry, 270(38), 22218-22222.