Anti-NPM1 (clone W24V156) Recombinant Antibody Coupled Liposome (VS-1024-FY157) (CAT#: VS-1024-FY157)

• Potential Clinical Applications
• Leukaemia

• Clone
• W24V156

• Antibody Type
• IgG1, kappa

• Antibody Host
• Mouse

• Antibody Reactivity
• Human

• Antibody Description
• This is a recombinant mouse monoclonal antibody specifically engineered to recognize and bind to nucleophosmin (NPM1), a protein that plays a crucial role in various cellular processes, including ribosome biogenesis, cell cycle regulation, and stress response. The antibody's design focuses on the unique epitope present on the NPM1 protein, ensuring high specificity and affinity for this target.

• Storage
• See in the COA

• Storage Shelf Time
• See in the COA

• Target
• NPM1

• Full Name
• Nucleophosmin 1

• Sequence Similarities
• Belongs to the nucleoplasmin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
  ADP-ribosylated.
  Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
  Sumoylated by ARF.
  Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36 (PubMed:19208757).

• Protein Refseq
• NP_001032827.1; NP_002511.1; NP_954654.1

• Function
• Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
  May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

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