Rabbit Anti-NPM1 Antibody (VS-0924-YC82) - Cancer Stem Cell Marker (CAT#: VS-0924-YC82)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human NPM1

• Species Reactivity
• Human

• Applications
• IHC, WB, IF

• Clonality
• Polyclonal

• Purification
• Affinity purified using the PrEST antigen as affinity ligand

• Concentration
• 0.1 mg/mL

• Buffer
• PBS, pH 7.2, with 40% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry (IHC): 1/1000-1/2500
  Western Blot (WB): 0.1-0.5 µg/mL
  Immunofluorescence (IF): 0.5-2 µg/mL

• Alternative Names
• B23; NPM

• Gene ID
• 4869

• UniProt ID
• P06748

• Long Name
• Nucleophosmin (nucleolar phosphoprotein B23, numatrin)

• Sequence Similarities
• Belongs to the nucleoplasmin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
  ADP-ribosylated.
  Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
  Sumoylated by ARF.
  Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36 (PubMed:19208757).

• Protein Refseq
• NP_001032827.1; NP_002511.1; NP_954654.1

• Function
• Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
  May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

• MOB-0264MC
• Goat Anti-Rabbit IgG Antibody (HRP)

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