Recombinant Anti-human NPM1 Antibody scFv Fragment
CAT#: MOB-711-S(P)
Recombinant Mouse Antibody scFv Fragment recognizes and reacts with Human NPM1, expressed in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/108_B11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/28259_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181163-NPM1
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Specifications
- Immunogen
- Human nucleophosmin (nucleolar phosphoprotein B23, numatrin)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- scFv
- Specificity
- Tested positive against native Human nucleophosmin (nucleolar phosphoprotein B23, numatrin)
- Species Reactivity
- Human
- Applications
- Antibody assay: Western blot; Immunohistochemistry; Functional Study
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-711 | Recombinant Anti-human NPM1 Antibody | ELISA, WB, IP, FuncS | IgG |
| ZG-0319C | Mouse Anti-NPM1 Recombinant Antibody (ZG-0319C) | WB, ICC | Mouse IgG |
| VS3-CJ453 | Mouse Anti-NPM1 Recombinant Antibody (clone ABT-NPM) | WB, IHC-P | Mouse IgG1, κ |
| VS4-CJ58 | Recombinant Mouse Anti-NPM1 Antibody (VS4-CJ58) | ELISA, WB, ICC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-711-F(E) | Recombinant Anti-human NPM1 Antibody Fab Fragment | ELISA, FuncS | Fab |
| MHH-711-F(E) | Recombinant Human Anti-human NPM1 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-711-S(P) | Recombinant Human Anti-human NPM1 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2480 | Hi-Affi™ Recombinant Rabbit Anti-NPM1 Monoclonal Antibody (DS2480AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF593 | A*02:01/Human NPM1 (AIQDLCLAV) MHC Pentamer | FCM | |
| MHC-YF594 | A*02:01/Human NPM1 (AIQDLCVAV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC431 | AbPlus™ Anti-NPM1 Magnetic Beads (VS-0724-YC431) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC82 | Rabbit Anti-NPM1 Antibody (VS-0924-YC82) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY346 | Mouse Anti-NHP NPM1 Recombinant Antibody (clone 7H10B9) | WB, IHC, IF, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1511 | Anti-NPM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4922 | Anti-Mouse NPM1 Immunohistochemistry Kit | IHC | |
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