Rabbit Anti-NR3C1 (pS226) Recombinant Antibody (clone R02-1H0)
CAT#: VS3-WK2028
This product is a rabbit recombinant antibody that is specific to phosphorylated NR3C1. The target of this antibody is phospho-NR3C1 (Ser226). The clone R02-1H0 can be used in WB analysis at a dilution of 1/500-1/1000 and IHC staining at a dilution of 1/50-1/100. The molecular weight of NR3C1 (pSer226) observed in WB experiments using this antibody is 94 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
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WB
Figure 1 Rabbit Anti-NR3C1 (pSer226) Recombinant Antibody (clone R02-1H0) in WB.
Western blot analysis of phospho-Glucocorticoid Receptor (Ser226) in Hela, A549, C6 lysates using anti-NR3C1 (pSer226) antibody, Cat# VS3-WK2028.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser226 of human Glucocorticoid Receptor
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser226 of human and rat NR3C1
- Species Reactivity
- Human, Rat
- Clone
- R02-1H0
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- Nuclear Receptor Subfamily 3 Group C Member 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Glucocorticoid Receptor; GRL; GR; Nuclear Receptor Subfamily 3, Group C, Member 1
- Gene ID
- 2908
- UniProt ID
- P04150
- Long Name
- Nuclear Receptor Subfamily 3 Group C Member 1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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