CytoStream™ Mouse Anti-NR3C1 Recombinant Antibody (VS13-YC840) (CAT#: VS13-YC840)
The CytoStream™ Mouse Anti-NR3C1 Recombinant Antibody is specific for human and mouse NR3C1. NR3C1 encodes the glucocorticoid receptor, a transcription factor regulating inflammatory responses and proliferation, with mutations causing glucocorticoid resistance. Alternative splicing and translation produce functional variants. The anti-NR3C1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Figure 3 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Figure 6 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
Specifications
- Immunogen
- Recombinant full-length human glucocorticoid receptor alpha.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human and Mouse NR3C1
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, FC, ICC, IF
- Conjugate
- NONE
- Related Disease
- Glucocorticoid Resistance, Generalized and Conn's Syndrome
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Immunocytochemistry (ICC): 1/50
Target
- Alternative Names
- Nuclear Receptor Subfamily 3 Group C Member 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Glucocorticoid Receptor; GRL; GR; Nuclear Receptor Subfamily 3, Group C, Member 1;
- Gene ID
- 2908
- UniProt ID
- P04150
- Long Name
- Nuclear Receptor Subfamily 3 Group C Member 1
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_000167.1; NP_001018084.1; NP_001018085.1; NP_001018086.1; NP_001018087.1
- Function
- Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1761z | Mouse Anti-NR3C1 Recombinant Antibody (clone 20F1) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1893-CN | Rabbit Anti-NR3C1 Polyclonal Antibody (MRO-1893-CN) | WB, IF | Rabbit IgG |
| ZG-0489U | Rabbit Anti-NR3C1 Recombinant Antibody (clone 2D8) | ELISA, WB, FC | Rabbit IgG |
| VS3-WK398 | Rabbit Anti-NR3C1 Recombinant Antibody (clone JF0952) | WB, FC | Rabbit IgG |
| VS3-XY1182 | Mouse Anti-NR3C1 Recombinant Antibody (clone 6E6) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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