Rabbit Anti-NR3C1 Recombinant Antibody (clone R02-5A3)
CAT#: VS3-FY1960
The R02-5A3 recombinant monoclonal antibody is specifically designed to target human NR3C1. It cross-reacts with mouse, rat, hamster. This glucocorticoid receptor antibody is guaranteed for western blot analysis of glucocorticoid receptor in 3T3, Raw264.7 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
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WB
Figure 1 Anti-NR3C1 Antibody (VS3-FY1960) in WB.
The Glucocorticoid Receptor expression in 3T3, Raw264.7 lysates were detected using the anti-Glucocorticoid Receptor by western blot assay.
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Specifications
- Immunogen
- Recombinant human glucocorticoid receptor protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster NR3C1
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R02-5A3
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 94 kDa.
- Related Disease
- Glucocorticoid Resistance, Generalized and Conn's Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Nuclear Receptor Subfamily 3 Group C Member 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Glucocorticoid Receptor; GRL; GR; Nuclear Receptor Subfamily 3, Group C, Member 1
- Gene ID
- 2908
- UniProt ID
- P04150
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus
- Post Translation Modifications
- Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity. (P04150-GCR_HUMAN)
Increased proteasome-mediated degradation in response to glucocorticoids (PubMed:11555652).
Isoform Alpha-B appears to be more susceptible to proteolytic degradation than isoform Alpha (PubMed:11435610).
(P04150-GCR_HUMAN)
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid.
The Ser-203, Ser-226 and Ser-404-phosphorylated forms are mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear (PubMed:12000743, 18838540).
Phosphorylation at Ser-211 increases transcriptional activity (PubMed:12000743, 18483179).
Phosphorylation at Ser-203, Ser-226 and Ser-404 decreases signaling capacity (PubMed:12000743, 18483179, 18838540).
Phosphorylation at Ser-404 may protect from glucocorticoid-induced apoptosis (PubMed:18838540).
Phosphorylation at Ser-203 and Ser-211 is not required in regulation of chromosome segregation (PubMed:25847991).
May be dephosphorylated by PPP5C, attenuates NR3C1 action ().
- Protein Refseq
- NP_000167.1; NP_001018084.1; NP_001018085.1; NP_001018086.1; NP_001018087.1
- Function
- Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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