Rabbit Anti-NR3C1 Recombinant Antibody (clone R02-5A3) (CAT#: VS3-FY1960)

• Immunogen
• Recombinant human glucocorticoid receptor protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat, Hamster NR3C1

• Species Reactivity
• Human, Mouse, Rat, Hamster

• Clone
• R02-5A3

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 94 kDa.

• Related Disease
• Glucocorticoid Resistance, Generalized and Conn's Syndrome

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Nuclear Receptor Subfamily 3 Group C Member 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Glucocorticoid Receptor; GRL; GR; Nuclear Receptor Subfamily 3, Group C, Member 1

• Gene ID
• 2908

• UniProt ID
• P04150

• Sequence Similarities
• Belongs to the nuclear hormone receptor family. NR3 subfamily.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

• Post Translation Modifications
• Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity. (P04150-GCR_HUMAN)
  Increased proteasome-mediated degradation in response to glucocorticoids (PubMed:11555652).
  Isoform Alpha-B appears to be more susceptible to proteolytic degradation than isoform Alpha (PubMed:11435610).
  (P04150-GCR_HUMAN)
  Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid.
  The Ser-203, Ser-226 and Ser-404-phosphorylated forms are mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear (PubMed:12000743, 18838540).
  Phosphorylation at Ser-211 increases transcriptional activity (PubMed:12000743, 18483179).
  Phosphorylation at Ser-203, Ser-226 and Ser-404 decreases signaling capacity (PubMed:12000743, 18483179, 18838540).
  Phosphorylation at Ser-404 may protect from glucocorticoid-induced apoptosis (PubMed:18838540).
  Phosphorylation at Ser-203 and Ser-211 is not required in regulation of chromosome segregation (PubMed:25847991).
  May be dephosphorylated by PPP5C, attenuates NR3C1 action ().

• Protein Refseq
• NP_000167.1; NP_001018084.1; NP_001018085.1; NP_001018086.1; NP_001018087.1

• Function
• Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody