Rabbit Anti-NUP50 Recombinant Antibody (clone R02-3H1)
CAT#: VS3-FY2054
The R02-3H1 recombinant monoclonal antibody exhibits specific targeting for human NUP50. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, ICC/IF. The binding specificity of the NUP50 antibody was determined using western blot analysis of NUP50 in Hela, C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-NUP50 Antibody (VS3-FY2054) in WB.
The NUP50 expression in Hela, C6 lysates were detected using the anti-NUP50 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47162/767_F11_1_selected.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47162/109446_B_6_4.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47162/109446_A_9_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47162/109446_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47162/109446_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000093000-NUP50
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Specifications
- Immunogen
- Synthetic peptide of human NUP50
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NUP50
- Species Reactivity
- Human, Rat
- Clone
- R02-3H1
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Lethal Congenital Contracture Syndrome 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Nucleoporin 50; 50 KDa Nucleoporin; Nucleoporin 50kDa; Nucleoporin Nup50; NPAP60L; Nuclear Pore-Associated Protein 60 KDa-Like
- Gene ID
- 10762
- UniProt ID
- Q9UKX7
- Cellular Localization
- Membrane, Nuclear pore complex, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys59, Lys62, Lys83, Lys127, Lys192, Lys229, and Lys258 (NX_Q9UKX7 [NX_Q9UKX7-1])
Modification sites at PhosphoSitePlus (Q9UKX7)
Glycosylation from GlyGen (Q9UKX7) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_006724166.1; XP_011528135.1; XP_024307914.1; XP_047297027.1; XP_047297028.1
- Function
- Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NUP50"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2525 | Hi-Affi™ Recombinant Rabbit Anti-NUP50 Monoclonal Antibody (DS2525AB) | WB, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC852 | Rabbit Anti-NUP50 Recombinant Antibody (VS13-YC852) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1532 | Anti-NUP50 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5054 | Anti-Mouse NUP50 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5053 | Anti-Human NUP50 Immunohistochemistry Kit | IHC |
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