Rabbit Anti-NUP50 Recombinant Antibody (clone R02-3H1) (CAT#: VS3-FY2054)

The R02-3H1 recombinant monoclonal antibody exhibits specific targeting for human NUP50. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, ICC/IF. The binding specificity of the NUP50 antibody was determined using western blot analysis of NUP50 in Hela, C6 lysates.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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  • Tested Data
  • Gene Expression
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  • COA
WB

Figure 1 Anti-NUP50 Antibody (VS3-FY2054) in WB.

Figure 1 Anti-NUP50 Antibody (VS3-FY2054) in WB.

The NUP50 expression in Hela, C6 lysates were detected using the anti-NUP50 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human NUP50
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat NUP50
  • Species Reactivity
  • Human, Rat
  • Clone
  • R02-3H1
  • Applications
  • Western Blot, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 50 kDa.
  • Related Disease
  • Lethal Congenital Contracture Syndrome 1

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Nucleoporin 50; 50 KDa Nucleoporin; Nucleoporin 50kDa; Nucleoporin Nup50; NPAP60L; Nuclear Pore-Associated Protein 60 KDa-Like
  • Cellular Localization
  • Membrane, Nuclear pore complex, Nucleus
  • Post Translation Modifications
  • Ubiquitination at Lys59, Lys62, Lys83, Lys127, Lys192, Lys229, and Lys258 (NX_Q9UKX7 [NX_Q9UKX7-1])
    Modification sites at PhosphoSitePlus (Q9UKX7)
    Glycosylation from GlyGen (Q9UKX7) 5 sites, 1 O-linked glycan (5 sites)
  • Function
  • Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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