Mouse Anti-P2RY1 Recombinant Antibody (clone 4G5B7)
CAT#: VS7-0425-WR537
This product is a mouse IgG2b antibody that binds to human P2RY1. The antibody 4G5B7 can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human placenta
High expression. RNA expression: 25.4 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_1_7_val_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Low expression. RNA expression: 0.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_5_1_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_9_4.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15577/35694_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169860-P2RY1
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Specifications
- Immunogen
- Recombinant human P2RY1 (AA: extra mix) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human P2RY1
- Species Reactivity
- Human
- Clone
- 4G5B7
- Molecule Class
- G-Protein-Coupled Receptors (GPCRs)
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- P2RY1; purinergic receptor P2Y, G-protein coupled, 1; P2Y1; P2Y purinoceptor 1; ATP receptor; platelet ADP receptor; P2 purinoceptor subtype Y1
- Gene ID
- 5028
- UniProt ID
- P47900
- Long Name
- Purinergic Receptor P2Y, G-Protein Coupled, 1
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_002554.1
- Function
- G-protein coupled receptor; Receptor; Transducer
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "P2RY1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-745 | Recombinant Anti-human P2RY1 Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-745 | Recombinant Human Anti-human P2RY1 Antibody | ELISA, FacS, FuncS | IgG |
| VS7-0425-WR536 | Mouse Anti-P2RY1 Recombinant Antibody (clone 4G5D6) | WB, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-745-F(E) | Recombinant Anti-human P2RY1 Antibody Fab Fragment | ELISA, FuncS | Fab |
| MHH-745-F(E) | Recombinant Human Anti-human P2RY1 Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-745-S(P) | Recombinant Anti-human P2RY1 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| MHH-745-S(P) | Recombinant Human Anti-human P2RY1 Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5131 | Anti-P2RY1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5132 | Anti-Rat P2RY1 Immunohistochemistry Kit | IHC |
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