Mouse Anti-PAX5 Recombinant Antibody (clone 1A7)
CAT#: VS7-0425-WR566
This product is a mouse IgG1 antibody that binds to human PAX5. The antibody 1A7 recognizes specific amino acid domain of human PAX5 (AA: 235-382). It can be used for WB assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/2055_D7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68498/1354_G10_4_selected.jpg
Normal Tissue
Figure 3 IHC staining of human lymph node
High expression. RNA expression: 39.7 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_8_8_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebral cortex
Low expression. RNA expression: 0.5 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_8_5_val_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_7_5.jpg
Normal Tissue
Figure 6 Spleen
Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Daudi, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196092-PAX5
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Specifications
- Immunogen
- Recombinant human PAX5 (AA: 235-382) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAX5
- Species Reactivity
- Human
- Clone
- 1A7
- Molecule Class
- Transcription Factor
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Leukemia, acute lymphoblastic, 3 (ALL3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PAX5; paired box 5; ALL3; BSAP; paired box protein Pax-5; paired domain gene 5; paired box homeotic gene 5; transcription factor PAX 5; B-cell lineage specific activator
- Gene ID
- 5079
- UniProt ID
- Q02548
- Long Name
- Paired Box 5
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001267476.1
- Function
- Developmental protein; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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