Mouse Anti-PAX5 Recombinant Antibody (clone 1A7) (CAT#: VS7-0425-WR566)
This product is a mouse IgG1 antibody that binds to human PAX5. The antibody 1A7 recognizes specific amino acid domain of human PAX5 (AA: 235-382). It can be used for WB assay.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/2055_D7_2_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/2055_D7_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68498/1354_G10_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68498/1354_G10_4_selected.jpg
Figure 3 IHC staining of human lymph node
(High expression. RNA expression: 39.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_8_8_val_selected.jpg
(High expression. RNA expression: 39.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_8_8_val_selected.jpg
Figure 4 IHC staining of human cerebral cortex
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_8_5_val_selected.jpg
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_8_5_val_selected.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_7_5.jpg
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_7_5.jpg
Figure 6 Spleen
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_9_4.jpg
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_9_4.jpg
Figure 7 RNA cell line category: Group enriched (Daudi, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196092-PAX5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196092-PAX5
Specifications
- Immunogen
- Recombinant human PAX5 (AA: 235-382) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAX5
- Species Reactivity
- Human
- Clone
- 1A7
- Molecule Class
- Transcription Factor
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Leukemia, acute lymphoblastic, 3 (ALL3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PAX5; paired box 5; ALL3; BSAP; paired box protein Pax-5; paired domain gene 5; paired box homeotic gene 5; transcription factor PAX 5; B-cell lineage specific activator
- Gene ID
- 5079
- UniProt ID
- Q02548
- Long Name
- Paired Box 5
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001267476.1
- Function
- Developmental protein; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-752-F(E) | Recombinant Anti-human PAX5 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-752-F(E) | Recombinant Human Anti-human PAX5 Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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