Rabbit Anti-PBK (pT9) Recombinant Antibody (clone R21-1A-8) (CAT#: VS3-WK2078)
This product is a rabbit recombinant antibody that is specific to phosphorylated PBK. The target of this antibody is phospho-PBK (Thr9). The clone R21-1A-8 can be used in WB analysis at a dilution of 1/500-1/1000 and IHC staining at a dilution of 1/100-1/200. The molecular weight of PBK (pThr9) observed in WB experiments using this antibody is 40 kDa.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Figure 4 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr9 of human PBK
- Species Reactivity
- Human
- Clone
- R21-1A-8
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
Target
- Alternative Names
- PDZ Binding Kinase; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; Nori-3; CT84; TOPK
- Gene ID
- 55872
- UniProt ID
- Q96KB5
- Long Name
- PDZ Binding Kinase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1560CT | Recombinant Mouse anti-Human PBK Monoclonal antibody (EML1820) | WB | |
| VS3-WK24 | Mouse Anti-PBK Recombinant Antibody (clone E11-C8) | WB, ELISA | Mouse IgG2b |
| VS3-FY1102 | Recombinant Rabbit Anti-PBK Antibody (clone R07-0B0) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY1816 | Rabbit Anti-PBK Recombinant Antibody (clone R05-1B6) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC872 | Rabbit Anti-PBK Recombinant Antibody (VS13-YC872) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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