Rabbit Anti-PBK (pT9) Recombinant Antibody (clone R21-1A-8)
CAT#: VS3-WK2078
This product is a rabbit recombinant antibody that is specific to phosphorylated PBK. The target of this antibody is phospho-PBK (Thr9). The clone R21-1A-8 can be used in WB analysis at a dilution of 1/500-1/1000 and IHC staining at a dilution of 1/100-1/200. The molecular weight of PBK (pThr9) observed in WB experiments using this antibody is 40 kDa.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr9 of human PBK
- Species Reactivity
- Human
- Clone
- R21-1A-8
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "PBK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-427 | Recombinant Human Anti-Human PBK Antibody, clone D532 | ELISA, IP-MS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1560CT | Recombinant Mouse anti-Human PBK Monoclonal antibody (EML1820) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2606 | Hi-Affi™ Recombinant Rabbit Anti-PBK Monoclonal Antibody (DS2606AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX851 | Mouse Anti-PBK Recombinant Antibody (clone 4F8-C6-A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK24 | Mouse Anti-PBK Recombinant Antibody (clone E11-C8) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1102 | Recombinant Rabbit Anti-PBK Antibody (clone R07-0B0) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1572 | Anti-PBK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5218 | Anti-Mouse PBK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5217 | Anti-Human PBK Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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