Recombinant Rabbit Anti-PBK Antibody (clone R07-0B0) (CAT#: VS3-FY1102)
This product is a recombinant rabbit antibody that recognizes PBK. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R07-0B0 is specific for human, rat PBK. The antigen is human pbk/spk recombinant protein.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Figure 4 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
Specifications
- Immunogen
- Human PBK/SPK recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat PBK
- Species Reactivity
- Human, Rat
- Clone
- R07-0B0
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 40 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PDZ Binding Kinase; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; Nori-3; CT84; TOPK
- Gene ID
- 55872
- UniProt ID
- Q96KB5
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1560CT | Recombinant Mouse anti-Human PBK Monoclonal antibody (EML1820) | WB | |
| VS3-QX851 | Mouse Anti-PBK Recombinant Antibody (clone 4F8-C6-A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-WK24 | Mouse Anti-PBK Recombinant Antibody (clone E11-C8) | WB, ELISA | Mouse IgG2b |
| VS3-FY1816 | Rabbit Anti-PBK Recombinant Antibody (clone R05-1B6) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC872 | Rabbit Anti-PBK Recombinant Antibody (VS13-YC872) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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