Rabbit Anti-PBK Recombinant Antibody (clone R05-1B6) (CAT#: VS3-FY1816)
The R05-1B6 is a rabbit recombinant monoclonal antibody that with high affinity for human PBK. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R05-1B6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Specific Inquiry
IHC
Figure 1 Anti-PBK Antibody (VS3-FY1816) in IHC.
Immunohistochemistry staining of paraffin-embedded mouse colon using the anti-PBK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PBK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PBK
- Species Reactivity
- Human, Mouse
- Clone
- R05-1B6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Central Corneal Ulcer and Perforated Corneal Ulcer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PDZ Binding Kinase; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; Nori-3; CT84; TOPK
- Gene ID
- 55872
- UniProt ID
- Q96KB5
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Post Translation Modifications
- Phosphorylated; in a cell-cycle dependent manner at mitosis. (Q96KB5-TOPK_HUMAN)
Ubiquitination at Lys8, Lys90, and Lys132 (NX_Q96KB5 [NX_Q96KB5-1])
Modification sites at PhosphoSitePlus (Q96KB5)
Glycosylation from GlyGen (Q96KB5) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001265874.1; NP_001349969.1; NP_060962.2
- Function
- Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-427 | Recombinant Human Anti-Human PBK Antibody, clone D532 | ELISA, IP-MS | IgG |
| VS3-QX851 | Mouse Anti-PBK Recombinant Antibody (clone 4F8-C6-A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-WK24 | Mouse Anti-PBK Recombinant Antibody (clone E11-C8) | WB, ELISA | Mouse IgG2b |
| VS3-FY1102 | Recombinant Rabbit Anti-PBK Antibody (clone R07-0B0) | WB, IHC-P, IP | Rabbit IgG |
| VS3-WK2078 | Rabbit Anti-PBK (pT9) Recombinant Antibody (clone R21-1A-8) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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