Rabbit Anti-PBK Recombinant Antibody (clone R05-1B6)
CAT#: VS3-FY1816
The R05-1B6 is a rabbit recombinant monoclonal antibody that with high affinity for human PBK. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R05-1B6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
❮
❯
IHC
Figure 1 Anti-PBK Antibody (VS3-FY1816) in IHC.
Immunohistochemistry staining of paraffin-embedded mouse colon using the anti-PBK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human PBK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PBK
- Species Reactivity
- Human, Mouse
- Clone
- R05-1B6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Central Corneal Ulcer and Perforated Corneal Ulcer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PDZ Binding Kinase; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; Nori-3; CT84; TOPK
- Gene ID
- 55872
- UniProt ID
- Q96KB5
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Post Translation Modifications
- Phosphorylated; in a cell-cycle dependent manner at mitosis. (Q96KB5-TOPK_HUMAN)
Ubiquitination at Lys8, Lys90, and Lys132 (NX_Q96KB5 [NX_Q96KB5-1])
Modification sites at PhosphoSitePlus (Q96KB5)
Glycosylation from GlyGen (Q96KB5) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001265874.1; NP_001349969.1; NP_060962.2
- Function
- Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1816. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PBK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-427 | Recombinant Human Anti-Human PBK Antibody, clone D532 | ELISA, IP-MS | IgG |
| MOB-1560CT | Recombinant Mouse anti-Human PBK Monoclonal antibody (EML1820) | WB | |
| VS3-QX851 | Mouse Anti-PBK Recombinant Antibody (clone 4F8-C6-A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-FY1102 | Recombinant Rabbit Anti-PBK Antibody (clone R07-0B0) | WB, IHC-P, IP | Rabbit IgG |
| VS3-WK2078 | Rabbit Anti-PBK (pT9) Recombinant Antibody (clone R21-1A-8) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2606 | Hi-Affi™ Recombinant Rabbit Anti-PBK Monoclonal Antibody (DS2606AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1572 | Anti-PBK Immunohistochemistry Kit | IHC | |
| VS-0525-XY5218 | Anti-Mouse PBK Immunohistochemistry Kit | IHC | |
| VS-0525-XY5217 | Anti-Human PBK Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, WB, FC
Application: ELISA, FuncS
-4.jpg)
Application: FC
Application: Neut, FuncS, IHC, ELISA, FC, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
