Rabbit Anti-PDK2 Recombinant Antibody (clone R01-5X9) (CAT#: VS3-XY3460)
The rabbit recombinant antibody specifically recognizes PDK2 (Pyruvate dehydrogenase kinase 2), which plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis. It specifically reacts with human, mouse and rat PDK2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/41_C4_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/41_C4_2_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_7_5.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_9_3.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_5_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_5_6.jpg
Figure 6 Skeletal muscle
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_3_6.jpg
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_3_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005882-PDK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005882-PDK2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5X9
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Autosomal Dominant Polycystic Kidney Disease; Polycystic Kidney Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PDHK2; PDKII
- Gene ID
- 5164
- UniProt ID
- Q15119
- Long Name
- Pyruvate dehydrogenase kinase 2
- Cellular Localization
- Mitochondrion matrix
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q15119)
Modification sites at neXtProt (NX_Q15119)
- Protein Refseq
- NP_001186828.1; NP_001186827.1
- Function
- Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2.
This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate.
Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
PPAR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2645 | Hi-Affi™ Recombinant Rabbit Anti-PDK2 Monoclonal Antibody (DS2645AB) | FC, ICC, IHC-P, WB | IgG |
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