Rabbit Anti-PDK2 Recombinant Antibody (clone R01-5X9)
CAT#: VS3-XY3460
The rabbit recombinant antibody specifically recognizes PDK2 (Pyruvate dehydrogenase kinase 2), which plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis. It specifically reacts with human, mouse and rat PDK2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/41_C4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_5_6.jpg
Normal Tissue
Figure 6 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_3_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005882-PDK2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5X9
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Autosomal Dominant Polycystic Kidney Disease; Polycystic Kidney Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PDHK2; PDKII
- Gene ID
- 5164
- UniProt ID
- Q15119
- Long Name
- Pyruvate dehydrogenase kinase 2
- Cellular Localization
- Mitochondrion matrix
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q15119)
Modification sites at neXtProt (NX_Q15119)
- Protein Refseq
- NP_001186828.1; NP_001186827.1
- Function
- Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2.
This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate.
Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism.
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Related Signaling Pathways
PPAR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
| MOB-1050MZ | Recombinant Mouse Anti-Human PDK2 Antibody | WB | Mouse antibody |
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| VS3-QX862 | Mouse Anti-PDK2 Recombinant Antibody (clone 2B8-C8-F6) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2645 | Hi-Affi™ Recombinant Rabbit Anti-PDK2 Monoclonal Antibody (DS2645AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1607 | Anti-PDK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5292 | Anti-Mouse PDK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5291 | Anti-Human PDK2 Immunohistochemistry Kit | IHC |
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