Mouse Anti-PECAM1 Monoclonal Antibody (VS7-0425-WR584) (CAT#: VS7-0425-WR584)
This product is a mouse IgG antibody that binds to human PECAM1. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4871/2005_B3_2_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4871/2005_B3_2_selected.jpg
Figure 2 Lung
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_2_4_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_2_4_val_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_8_5.jpg
Figure 6 RNA cell line category: Group enriched (HUVEC TERT2, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000261371-PECAM1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000261371-PECAM1
Specifications
- Immunogen
- Recombinant human PECAM1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PECAM1
- Species Reactivity
- Human
- Molecule Class
- Immunoglobulin
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Gastrointestinal stromal tumor (GIST); GIST-plus syndrome (GISTPS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PECAM1; Platelet/Endothelial Cell Adhesion Molecule 1; EndoCAM; CD31; Platelet/Endothelial Cell Adhesion Molecule; GPIIA; PECAM-1; Platelet Endothelial Cell Adhesion Molecule; PECA1
- Gene ID
- 5175
- UniProt ID
- P16284
- Long Name
- Platelet And Endothelial Cell Adhesion Molecule 1
- Cellular Localization
- Cell junction; Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Lipoprotein; Palmitate; Phosphoprotein
- Protein Refseq
- NP_000433.4
- Function
- Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684).; Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684).; Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302).; Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308).; Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892).; Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892).; Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896).; Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896).; Induces susceptibility to atherosclerosis (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1852CQ | Recombinant Mouse Anti-PECAM1 Antibody (HEC7) | BL, ELISA, FC, ICC/IF, IHC, IP, WB | IgG2a |
| NEUT-1855CQ | Recombinant Mouse Anti-PECAM1 Antibody (WM59) | FC, IHC, IF, BL | IgG1, κ |
| NEUT-1856CQ | Recombinant Rat Anti-Pecam1 Antibody (390) | FC, IP, IHC-Fr, ICC, BL | IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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