Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4) (CAT#: ZG-0509U)
Rabbit Anti-PELP1 Antibody (clone 6H4) displayed high specificity against the PELP1 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PELP1 Antibody (ZG-0509U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate
All lanes: PELP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120 kDa
Observed band size: 160 kDa
IHC
Figure 2 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PELP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PELP1
- Species Reactivity
- Human
- Clone
- 6H4
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Amelogenesis Imperfecta, Type Ig and Breast Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Proline-; glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3; PELP1; HMX3 MNAR
- Gene ID
- 27043
- UniProt ID
- Q8IZL8
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "PELP1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY3461 | Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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