Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4)
CAT#: ZG-0509U
Rabbit Anti-PELP1 Antibody (clone 6H4) displayed high specificity against the PELP1 protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-PELP1 Antibody (ZG-0509U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate
All lanes: PELP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120 kDa
Observed band size: 160 kDa
IHC
Figure 2 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53966/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_5_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_7_1.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141456-PELP1
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Specifications
- Immunogen
- A synthesized peptide derived from human PELP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PELP1
- Species Reactivity
- Human
- Clone
- 6H4
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Amelogenesis Imperfecta, Type Ig and Breast Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PELP1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY3461 | Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR594 | Mouse Anti-PELP1 Recombinant Antibody (clone 2D6D11) | FC | Mouse IgG2a |
| VS7-0425-WR595 | Mouse Anti-PELP1 Recombinant Antibody (clone 4F1D1) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1617 | Anti-PELP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5312 | Anti-Human PELP1 Immunohistochemistry Kit | IHC |
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