Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4) (CAT#: ZG-0509U)
Rabbit Anti-PELP1 Antibody (clone 6H4) displayed high specificity against the PELP1 protein of Human.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Rabbit Anti-PELP1 Antibody (ZG-0509U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate
All lanes: PELP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120 kDa
Observed band size: 160 kDa
IHC
Figure 2 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PELP1 Antibody (ZG-0509U) in IHC
IHC image of ZG-0509U diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PELP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PELP1
- Species Reactivity
- Human
- Clone
- 6H4
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Amelogenesis Imperfecta, Type Ig and Breast Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Proline-; glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3; PELP1; HMX3 MNAR
- Gene ID
- 27043
- UniProt ID
- Q8IZL8
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY3461 | Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR594 | Mouse Anti-PELP1 Recombinant Antibody (clone 2D6D11) | FC | Mouse IgG2a |
| VS7-0425-WR595 | Mouse Anti-PELP1 Recombinant Antibody (clone 4F1D1) | FC | Mouse IgG2b |
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