Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6)
CAT#: VS3-XY3461
The recombinant rabbit antibody is specific for PELP1 (Proline, glutamate and leucine rich protein 1), which plays a role in estrogen receptor (ER) genomic activity. The antibody specifically reacts with human PELP1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53966/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_5_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_7_1.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141456-PELP1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PELP1
- Species Reactivity
- Human
- Clone
- R08-7B6
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Breast Cancer; Amelogenesis Imperfecta, Type Ig
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- MNAR; P160
- Gene ID
- 27043
- UniProt ID
- Q8IZL8
- Long Name
- Proline, glutamate and leucine rich protein 1
- Cellular Localization
- Nucleus, Nucleoplasm
- Post Translation Modifications
- Transiently sumoylated, preferentially conjugated to SUMO2 or SUMO3.
Sumoylation causes nucleolar exclusion of PELP1 and promotes the recruitment of MDN1 to pre-60S particles.
Desumoylation by SUMO isopeptidase SENP3 is needed to release both PELP1 and MDN1 from pre-ribosomes.
(Q8IZL8-PELP1_HUMAN)
- Protein Refseq
- NP_055204.4; NP_001265170.1
- Function
- Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (PubMed:14963108).
Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner.
Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol.
Plays a role in E2-mediated cell cycle progression by interacting with RB1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PELP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0509U | Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4) | ELISA, WB, IHC | Rabbit IgG |
| VS7-0425-WR594 | Mouse Anti-PELP1 Recombinant Antibody (clone 2D6D11) | FC | Mouse IgG2a |
| VS7-0425-WR595 | Mouse Anti-PELP1 Recombinant Antibody (clone 4F1D1) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1617 | Anti-PELP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5312 | Anti-Human PELP1 Immunohistochemistry Kit | IHC |
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