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  • Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6)

Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6) (CAT#: VS3-XY3461)

The recombinant rabbit antibody is specific for PELP1 (Proline, glutamate and leucine rich protein 1), which plays a role in estrogen receptor (ER) genomic activity. The antibody specifically reacts with human PELP1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.


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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53966/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/ihc_selected.jpg
Figure 3 Lymph node
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_7_8.jpg
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_9_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_5_6.jpg
Figure 8 Skin
Figure 8 Skin
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_7_1.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141456-PELP1

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human PELP1
  • Species Reactivity
  • Human
  • Clone
  • R08-7B6
  • Applications
  • WB, IHC-P, ICC, IF, FC
  • Related Disease
  • Breast Cancer; Amelogenesis Imperfecta, Type Ig

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IF: 1/50-1/200
    FC: 1/50-1/100

Target

  • Alternative Names
  • MNAR; P160
  • Long Name
  • Proline, glutamate and leucine rich protein 1
  • Cellular Localization
  • Nucleus, Nucleoplasm
  • Post Translation Modifications
  • Transiently sumoylated, preferentially conjugated to SUMO2 or SUMO3.
    Sumoylation causes nucleolar exclusion of PELP1 and promotes the recruitment of MDN1 to pre-60S particles.
    Desumoylation by SUMO isopeptidase SENP3 is needed to release both PELP1 and MDN1 from pre-ribosomes.
    (Q8IZL8-PELP1_HUMAN)
  • Function
  • Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (PubMed:14963108).
    Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner.
    Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol.
    Plays a role in E2-mediated cell cycle progression by interacting with RB1.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PELP1"

Recombinant Antibody

CAT Product Name Application Type
ZG-0509U Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4) ELISA, WB, IHC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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