Rabbit Anti-PELP1 Recombinant Antibody (clone R08-7B6) (CAT#: VS3-XY3461)
The recombinant rabbit antibody is specific for PELP1 (Proline, glutamate and leucine rich protein 1), which plays a role in estrogen receptor (ER) genomic activity. The antibody specifically reacts with human PELP1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53966/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53966/if_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/ihc_selected.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_7_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_7_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_9_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_9_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_A_5_6.jpg
Figure 8 Skin
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_7_1.jpg
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60760/134465_B_7_1.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141456-PELP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141456-PELP1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PELP1
- Species Reactivity
- Human
- Clone
- R08-7B6
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Breast Cancer; Amelogenesis Imperfecta, Type Ig
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- MNAR; P160
- Gene ID
- 27043
- UniProt ID
- Q8IZL8
- Long Name
- Proline, glutamate and leucine rich protein 1
- Cellular Localization
- Nucleus, Nucleoplasm
- Post Translation Modifications
- Transiently sumoylated, preferentially conjugated to SUMO2 or SUMO3.
Sumoylation causes nucleolar exclusion of PELP1 and promotes the recruitment of MDN1 to pre-60S particles.
Desumoylation by SUMO isopeptidase SENP3 is needed to release both PELP1 and MDN1 from pre-ribosomes.
(Q8IZL8-PELP1_HUMAN)
- Protein Refseq
- NP_055204.4; NP_001265170.1
- Function
- Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (PubMed:14963108).
Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner.
Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol.
Plays a role in E2-mediated cell cycle progression by interacting with RB1.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0509U | Rabbit Anti-PELP1 Recombinant Antibody (clone 6H4) | ELISA, WB, IHC | Rabbit IgG |
| VS7-0425-WR594 | Mouse Anti-PELP1 Recombinant Antibody (clone 2D6D11) | FC | Mouse IgG2a |
| VS7-0425-WR595 | Mouse Anti-PELP1 Recombinant Antibody (clone 4F1D1) | FC | Mouse IgG2b |
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