Rabbit Anti-Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Antibody (clone 1A9) (CAT#: ZG-0458U)
Rabbit Anti-Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody (clone 1A9) displayed high specificity against the MAPK8/MAPK9/MAPK10 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody (ZG-0458U) in WB
Western Blot
Positive WB detected in 293 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 54 KDa
Observed band size: 46, 54 KDa
ICC
Figure 2 Rabbit Anti-Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody (ZG-0458U) in ICC
Immunocytochemistry analysis of ZG-0458U diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
- Species Reactivity
- Human
- Clone
- 1A9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hepatitis C, Hepatitis, Breast Cancer, Pheochromocytoma, Lennox-Gastaut Syndrome and Opioid Abuse.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; mitogen-activated protein kinase 8; JUN N-terminal kinase; MAP kinase 8; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; EC 2.7.11.24;; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA; mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; c-Jun N-terminal kinase 2; c-Jun kinase 2; stress-activated protein kinase 1a; stress-activated protein kinase JNK2; EC 2.7.11.24;; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK; mitogen-activated protein kinase 10; JNK3 alpha protein kinase; MAP kinase 10; MAP kinase p49 3F12; c-Jun N-terminal kinase 3; stress activated protein kinase beta; stress-activated protein kinase 1b; stress-activated protein kinase JNK3
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MAPK8/MAPK9/MAPK10"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0883-CN | Recombinant Rabbit Anti-MAPK8/MAPK9/MAPK10 Monoclonal Antibody (CBACN-329) | WB, IF, IP | Rabbit IgG |
| MRO-2342-CN | Recombinant Rabbit Anti-MAPK8/MAPK9/MAPK10 (phosphorylated Thr183+Thr183+Thr221) Monoclonal Antibody (CBACN-633) | WB, IF, IHC, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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