Rabbit Anti-Phospho-RPS6KA1 (S380) Recombinant Antibody (clone 1E11)
CAT#: ZG-0553U
Rabbit Anti-Phospho-RPS6KA1 (S380) Antibody (clone 1E11) displayed high specificity against the RPS6KA1 protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-Phospho-RPS6KA1 (S380) Antibody (ZG-0553U) in WB
Western Blot
Positive WB detected in A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate
All lanes Phospho-RPS6KA1 antibody at 1.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 KDa
Observed band size: 90 KDa
IP
Figure 2 Rabbit Anti-Phospho-RPS6KA1 (S380) Antibody (ZG-0553U) in IP
Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of ZG-0553U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0553U(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/21_H4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117676-RPS6KA1/subcellular
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-RPS6KA1 (S380)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-RPS6KA1 (S380)
- Species Reactivity
- Human
- Clone
- 1E11
- Applications
- ELISA, WB, IP
- Conjugate
- Unconjugated
- Related Disease
- Coffin-Lowry Syndrome and Tuberous Sclerosis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IP:1:200-1:1000
Target
- Alternative Names
- Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1; MAPKAPK1A; RSK1
- Gene ID
- 6195
- UniProt ID
- Q15418
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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