Mouse Anti-PLA2G3 Recombinant Antibody (clone 8G8)
CAT#: VS-0224-XY101
The product is a mouse monoclonal antibody that specifically binds human Phospholipase A2 Group III (PLA2G3). It was purified by protein G resins. It has been detected by Western blot and other applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/2063_A8_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside the reaction centra.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/ membranous
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranou
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51775_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/ membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (AF22, AN3-CA, NTERA-2, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100078-PLA2G3/subcellular
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Specifications
- Immunogen
- Amino acids 409-509 of human PLA2G3 (NP_056530) produced in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PLA2G3
- Species Reactivity
- Human
- Clone
- 8G8
- Applications
- Western Blot
- Related Disease
- Joubert Syndrome 23
Product Property
- Purification
- Protein G purified
- Buffer
- PBS, pH 7.3, with 1% BSA
- Preservative
- 0.02% Sodium azide, 50% Glycerol
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Western blot (1/2000).
Target
- Alternative Names
- PLA2G3; Phosphatidylcholine 2-Acylhydrolase GIII; GIII-SPLA2; Phospholipase A2, Group III; Phosphatidylcholine 2-Acylhydrolase 3; Group III Secreted Phospholipase A2; EC 3.1.1.4; SPLA2III; Group III Secretory Phospholipase A2; SPLA2-III; Group 3 Secretory
- Gene ID
- 50487
- UniProt ID
- Q9NZ20
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2570z | Mouse Anti-PLA2G3 Recombinant Antibody (clone 20G6) | WB, IHC, FuncS | Mouse IgG1 |
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