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    Mouse Anti-PLA2G3 Recombinant Antibody (clone 8G8) (CAT#: VS-0224-XY101)

    The product is a mouse monoclonal antibody that specifically binds human Phospholipase A2 Group III (PLA2G3). It was purified by protein G resins. It has been detected by Western blot and other applications.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line EFO-21
    Figure 1 IF staining of human cell line EFO-21
    (Immunofluorescent staining of human cell line EFO-21 shows localization to the Golgi apparatus.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/2063_A8_4_selected.jpg
    Figure 2 IHC staining of human lymph node
    Figure 2 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside the reaction centra.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/ membranous
    Glial cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranou)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51775_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/ membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining:

    Medium

    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining:

    Medium

    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_4_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/21016/51707_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Group enriched (AF22, AN3-CA, NTERA-2, SCLC-21H)
    Figure 9 RNA cell line category: Group enriched (AF22, AN3-CA, NTERA-2, SCLC-21H)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100078-PLA2G3/subcellular

    Specifications

    • Immunogen
    • Amino acids 409-509 of human PLA2G3 (NP_056530) produced in E. coli
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human PLA2G3
    • Species Reactivity
    • Human
    • Clone
    • 8G8
    • Applications
    • Western Blot
    • Related Disease
    • Joubert Syndrome 23

    Product Property

    • Purification
    • Protein G purified
    • Buffer
    • PBS, pH 7.3, with 1% BSA
    • Preservative
    • 0.02% Sodium azide, 50% Glycerol
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested in Western blot (1/2000).

    Target

    • Alternative Names
    • PLA2G3; Phosphatidylcholine 2-Acylhydrolase GIII; GIII-SPLA2; Phospholipase A2, Group III; Phosphatidylcholine 2-Acylhydrolase 3; Group III Secreted Phospholipase A2; EC 3.1.1.4; SPLA2III; Group III Secretory Phospholipase A2; SPLA2-III; Group 3 Secretory

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    IgG Antibody Products
    CAT Product Name Application Type
    MOB-2570z Mouse Anti-PLA2G3 Recombinant Antibody (clone 20G6) WB, IHC, FuncS Mouse IgG1

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