Mouse Anti-PODXL Recombinant Antibody (clone 4D2G7) (CAT#: ZG-0167U)

Figure 1 Mouse Anti-PODXL Antibody (ZG-0167U) in WB

Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue. All lanes: PODXL antibody at 1.28µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Figure 2 Mouse Anti-PODXL Antibody (ZG-0167U) in IHC

Immunohistochemistry of paraffin-embedded human melanoma using ZG-0167U at dilution of 1:200.

Figure 3 Mouse Anti-PODXL Antibody (ZG-0167U) in IF

Immunofluorescent analysis of Hela cells using ZG-0167U at a dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-PODXL Antibody (ZG-0167U) in FC

Overlay histogram showing Hela cells stained with ZG-0167U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Figure 5 Mouse Anti-PODXL Antibody (ZG-0167U) in IP

Immunoprecipitating PODXL in HEK293 whole cell lysate. Lane 1: Mouse control IgG (1µg) instead of ZG-0167U in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0167U (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)